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ELECTROPORATION

ELECTROPORATION. Agustina Setiawati. DEFINISI. Electro = muatan listrik Poration = pembentukan pori …so ELECTROPORATION adalah peningkatan konduktivitas dan permiabilitas membran sel akibat efek muatan listrik Pada satu saat , dapat menimbulkan pori2 pada membran sel

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ELECTROPORATION

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  1. ELECTROPORATION AgustinaSetiawati

  2. DEFINISI Electro = muatanlistrik Poration= pembentukanpori …so ELECTROPORATION adalahpeningkatankonduktivitasdanpermiabilitasmembranselakibatefekmuatanlistrik Padasatusaat, dapatmenimbulkan pori2 padamembransel Electroporation = Electropermiabilization

  3. Panas/Heat 42-65ºC selama 30 detik Muatanlistrik optimum 300-400 mV selama< 1 ms

  4. MekanismeElektroporasi Ketikamuatanlistrikdiberikan, ion-ion akanberadakonformasisptgambar Terbentukporidalammembransel, DNA masukdalamsel Muatanlistrikdihentikan, membranselkembalisptsemula

  5. IRE? Proseselektroporasiharusdiakhiridgnprosespendinginanutkmenghindari IRREVERSIBEL ELECTROPORATION (IRE)  selmati

  6. APLIKASI ELEKTROPORASI Elektroporasimeningkatkanekspresi gen dibandingkandgntanpaelektroporasi

  7. APLIKASI ELEKTROPORASI Elektroporasimeningkatkanresponimunvaksinantrak pd monyet

  8. Kelebihan Non-invasif Tidakmenggunakanbahankimia Tidakmengubahfungsidanstrukturmembransel Mudahdancepat Dapatdiaplikasikankesemuajenissel

  9. Kekurangan Transformasi non spesifik Kemungkinanterjadikerusakanmembransel yang berakibatkematiansel Adanya JOULE EFFECT (timbulnyapanasmenimbulkanefek IRE)

  10. THANK YOU

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