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mitochondria. Mitochondria in Kidney: Black staining of the mitochondria More black staining means more mitochondria Bottom of kidney slide lots = more transport Top has little = lighter = less transport Mitochondria have cristae

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slide2

Mitochondria in Kidney:

    • Black staining of the mitochondria
    • More black staining means more mitochondria
      • Bottom of kidney slide lots = more transport
      • Top has little = lighter = less transport
    • Mitochondria have cristae
    • Mitochondria are rod like and about 0.25 um in diameter (can be 7 um in length)
slide5

Ribosomes and RER in spinal nervous tissue

    • Cytoplasm is filled with this blue staining material = RER and Ribosomes
      • The stain actually is staining the ribosomal RNA in the ribosomes on the RER
      • The nucleolus is also highly stained because this is where ribosomes are constructed
      • ***Do not rely on color to make determinations because the parts being stained could be dispersed, concentrated, etc which will cause different coloring
        • Should probably understand what each stain marks/ binds to
slide7

Golgi apparatus in Dorsal Root Ganglion Slide

    • The Nucleus is a clear color
      • Surrounded by black dots which may look crescent shaped
        • These are the Golgi apparatuses
          • Golgi are perinuclear (only around the nucleus)
          • Can see very discretely unlike the ribosomes
            • Specific silver stain that only differentiates Golgi
slide9

Microfilaments and Skeletal Muscle

    • Some microfilaments stain dark and then adjacent to this there are other microfilaments that stain lighter which causes the appearance of bands
  •  Direction of Contraction
  • Striated tissue seen as light, vertical bands. Microfilaments actually run horizontally
  • A-Band, Seen as darker vertical band, composed of myosin. (Stationary during contraction)
  • All considered inclusions. Not membrane bound and low metabolic activity
slide14

Nuclei

    • Use nuclei to identifiy cell type
      • Use size, location, and shape to determine cell type
slide15

Nucleus and the Liver

    • Can see difference in the nucleus of the hepatocyte and the endothelial cell
slide17

Skeletal muscles and nuclei

    • Nuclui located on the periphery
    • Can have many nuclei
    • Also see microfilaments (pink) in muscle cell
slide18

Blastula and Mitosis (Nucleus examination)

    • Black dots are yolk in blastula  ignore it
unit 1 exam microanatomy lab practical practice questions

Unit 1 Exam Microanatomy Lab Practical Practice Questions

Cytology, Epithelium, and Connective Tissue

slide23

What membrane bound organelle indicated by the blue arrows appears as these dark moon shaped structures in this type of staining and is involved in packaging of proteins into vesicles?

slide26

What is the organelle that is show in this Micrograph that is involved in the production of protein?

slide28

These cytoplasmic granules indicated by the green arrows are utilized by the mitochondria because they supply energy in the form of what?

slide29

This double membraned organelle regulates signals to the entire cell what structures indicated by the red and green arrows allow them send out these signals?