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Analysis of VEGF, PlGF, and sFlt-1 Levels in Wild Type and Mutant Models

This study investigates the levels of Vascular Endothelial Growth Factor (VEGF), Placental Growth Factor (PlGF), and soluble Flt-1 (sFlt-1) in different genetic models, differentiating between wild type (WT) and mutant (sFlt-1+/-; sFlt-1-/−) configurations. By quantifying these biomarkers in ng/ml and pg/ml, we aim to understand their regulatory roles in angiogenesis under varying conditions. The results highlight significant variations in growth factor expression that may influence therapeutic strategies in vascular-related disorders.

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Analysis of VEGF, PlGF, and sFlt-1 Levels in Wild Type and Mutant Models

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  1. a b 0.4 50 40 0.3 30 * # sFlt-1 (ng /ml) sFlt-1/GAPDH 0.2 * * 20 # 0.1 10 * 0 0 WT sFlt-1-/- sFlt-1+/- sFlt-1-/- sFlt-1+/- WT c d 40 1.5 30 1.0 PlGF( pg /ml) PlGF/GAPDH 20 0.5 10 0 0 sFlt-1+/- WT sFlt-1-/- sFlt-1+/- WT sFlt-1-/- e f 0.4 20 0.3 15 0.2 VEGF/GAPDH VEGF (pg /ml) 10 0.1 5 0 0 WT sFlt-1-/- sFlt-1+/- sFlt-1+/- WT sFlt-1-/-

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