Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods
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Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods. Beatriz Díez 1 , Terence L. Marsh 2 , Ramon Massana 1 and Carlos Pedrós-Alió 1

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Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Beatriz Díez1, Terence L. Marsh2 , Ramon Massana1 and Carlos Pedrós-Alió1

1Departament de Biologia Marina, Institut de Ciències del Mar, CSIC, Passeig Joan de Borbó s/n, E-08039 Barcelona, Catalunya, Spain

2Center of Microbial Ecology and Department of Microbiology, 41 Gilner Hall, Michigan State University, East Lansing, 48824 Michigan, USA


Southern Ocean: area studied by two fingerprintins methods


DHARMA cruise by two fingerprintins methods

DOVETAIL cruise


Objectives by two fingerprintins methods

Study the distribution and variability of eukaryotic picoplankton in Southern Ocean

Comparison of DGGE and T-RFLP results

Identification of the dominant populations by both techniques

Emphasis on the distribution of novel phylogenetic groups: stramenopiles and alveolates


ICE-EDGE by two fingerprintins methods

DOVETAIL transect

DHARMA transect

EDGE-ICE


DOVETAIL Surface by two fingerprintins methods

T-RFLP (MspI)

Mantoniella

DOV1

DOV2

DOV3

DOV4

Phaeocystis

DOV5

DOV6


DOVETAIL transect by two fingerprintins methods

DGGE

T-RFLP (MspI and RsaI)

ICE-EDGE

ICE

-EDGE

A=SURFACE

B=Bottom of mixed layer


19** by two fingerprintins methods

18

17**

15

16

14

10

13

9

12

11

8

7*

6

5

4

3

2

1


Edge-ice by two fingerprintins methods

SAF

DH32

DH12

DH18

DH24

DH1

Dharma 5 - 0.2µm

DH1= Ice-edge

DH12= Scotia Front

DH24= Polar Front

DH32= Subantartic Front


DHARMA by two fingerprintins methods

DGGE

T-RFLP (MSPI)

ICE

ICE

WSF

ICE

WSF

ACC

ACC

WSF

PF

PF AND SAF

SAF


DHARMA CYTOMETRY by two fingerprintins methods

DH11 ---

+-----

DH14 --- |

+-----

DH18 -- | |

+-- | |

DH12 -- | | |

+---- |

DH5 - | |

+- | |

DH9 -| | |

+-- |

DH20 -- |

+-------------------------------

DH22 -- |

+-----------

DH3 -+

|

DH1 --

DH24 ------

+--------------------------------------

DH28 - |

| |

DH26 + |

| |

DH32 + |

+-----

DH30 -

PF


*Novel alveolates by two fingerprintins methods

**Novel stramenopiles


DH18 (WSF) by two fingerprintins methods

19**

18

17**

16

10

13

12

11

8

7*

6

5

4*

10 25 60 100 250 500 1000 2000 3000

Depth (meters)


Conclusions by two fingerprintins methods

DGGE and T-RFLP fingerprinting approaches provide information about changes in population distribution. Also they offer the possibility to identify the dominant components of the natural populations.

When the same size fractions were compared, the assemblages were rather similar in the two transects.

Despite the high resolution of the T-RFLP technique, usually more than one species had the same restriction site in the 18S rDNA, resulting in an identical peak size. The most likely candidate must be decided with information from other techniques


Although diatoms have been usually found to be dominant in Antarctic algal blooms and are the best known group, it is clear that small-celled species (<5 µm) and not only autotrophic organisms, are very important in many antarctic locations

Similar phylogenetic groups and dominant species were detected by DGGE and T-RFLP.

Prasinophyceae, Prymnesiophyceae, Bacillariophyceae, Novel stramenopiles, Dinophyceae, and Novel alveolates, were found to be the most widespread groups, further supporting the suggestion that these organisms are common and widely distributed both in Antarctic waters and in most Oceans


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