1 / 8

This reaction forms a cyclic intron and a short oligonucleotide.

5’ G. UUU. 5’ G. UUUpA 16 CCUpU 20 UUG. G 414 -OH. OH 3’. A 16 CCUpU 20 UUG. p. G 414. This reaction forms a cyclic intron and a short oligonucleotide. 5’ A 16 CCUpU 20 UUG. G 414 -OH. Hydrolysis gives a linear form of the intron called L-15. G414 then attacks the 5’ side of U20.

kirby
Download Presentation

This reaction forms a cyclic intron and a short oligonucleotide.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 5’ G UUU 5’ G UUUpA16CCUpU20UUG G414 -OH OH 3’ A16CCUpU20UUG p G414 This reaction forms a cyclic intron and a short oligonucleotide.

  2. 5’ A16CCUpU20UUG G414 -OH Hydrolysis gives a linear form of the intron called L-15. G414 then attacks the 5’ side of U20. L-19 RNA is formed.

  3. L-19 RNA still has an IGS and a reactive G414. L-19 is a ribozyme -it catalyses reactions that make or break phosphodiester bonds.

  4. G-OH 5’ -p-CCCCC-OH GGGAGG-5’ Catalytic activities of L-19 RNAs 1. PolyC polymerase A C5 oligonucleotide binds the IGS. The 3’ terminal residue is transferred to G414.

  5. Another C5 oligonucleotide binds and is extended to C6. G-pC-OH 5’ -p-CCCCC-OH 5’ -p-CCCCCC-OH GGGAGG--5’ Repeated cycles generate longer polynucleotides.

  6. 2. Sequence-specific endoribonuclease. The IGS is engineered to base pair with the target RNA sequence.A free G residue cleaves the target. G-OH 5’ -CCCUCU 3’ GGGAGG--5’ 5’-CCCUCU-OH + 5’ G 3’

  7. 5’ Y-OH 3. RNA ligase. The 5’ G of one RNA occupies the G binding site. The other RNA base-pairs with the IGS. Ligation results. Gp XXXXXXXX--5’ 5’ Y 3’ + G

  8. 5’ UCU p 4. Phosphatase. G-OH GGGAGG 5’

More Related