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Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,

Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa). Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong, Gu¨nter Theißen,* and Zheng Meng*. 報告者 : 劉育羣 N99H0011 專討老師 : 鄭伯智 林宏榮 醫師 日期 : 05/08/2011.

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Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong,

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  1. Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa) Rongfeng Cui, Jiakun Han, Suzhen Zhao, Kunmei Su, Feng Wu, Xiaoqiu Du, Qijiang Xu, Kang Chong, Gu¨nter Theißen,* and Zheng Meng* 報告者:劉育羣 N99H0011 專討老師:鄭伯智 林宏榮 醫師 日期:05/08/2011

  2. Zea mays玉米 Hordeum vulgare大麥 Triticum aestivum小麥 糧食作物中包括許多禾本科植物 Oryza sativa水稻 Sorghum bicolor蜀黍

  3. 中文名稱：水稻 • 英文名稱：Rice • 學   名：Oryza sativa L. • 科名：禾本科(Gramineae) • 別   名：稻仔 • 原產地：中國長江流域。1973年在浙江省餘姚縣首次發現約7000年前長江下游地區河姆渡文化遺址中有大量的稻穀遺存，證明當時已有農業生產，已開始種植水稻。 • 分布：台灣全島中低海拔水源充足，灌溉方便之平原。 • 用途： 稻米以碾成白米供食用為主，是人類主食，次可釀酒，並可製造米粉、米麩、蘿蔔糕等。 • 特  性：水稻是台灣最重要的農作物，也是亞洲人民食用的主食。水稻屬於禾本科單子葉的草本植物，喜歡溫暖多雨的氣候，台灣水稻一年可以栽種兩次，栽植水稻要先育秧苗，然後再移植到水田裏，約３至４個月可以收穫。我們吃的白米就是稻子的胚乳。

  4. Oryza sativa水稻 小穗由一朵小花組成，外由外穎及內穎包被，單生於穗柄上。 小穗→

  5. Outline • Introduction • Experimental Procedures • Results • Conclusions

  6. Introduction

  7. MADS-box Gene Family • The MADS-box family transcription factors play key roles in controlling development and signal transduction in eukaryotes. • Phylogenetic analyses split the MADS-box gene family into two classes, termed type I (SRF-like) and type II (MEF2-like) genes. Messenguy and Dubois (2003) Alvarez-Buylla et al. (2000) de Folter S and Angenent GC (2006)

  8. New Floral ABCE and Quartet Model Se – sepal (花萼) Pe – petal (花瓣) St – stamen (雄蕊) Ca – carpel (心皮) Soltis DE et al., (2007)

  9. Evolutionary implications of class E genes in rice The class E gene function specifying the identity of lodicules, stamens and carpels may require the direct interaction of OsMADS1 and OsMADS7/8 during spikelet meristem development. lemma(外穎)，stamen(雄蕊)，carpel(心皮)，palea(內穎)，lodicule(漿片)，sterile lemma(護穎)，pedicel(花梗)

  10. Experimental Procedures • Rice transformation • Scanning electron microscopy • In situ hybridization • RT-PCR and real-time PCR expression analysis • Yeast two-hybrid (Y2H) analyses • Coimmunoprecipitation (Co-IP) analyses of the rice SEP-like proteins in Arabidopsis mesophyll protoplasts

  11. Results

  12. Molecular characterization of RNAi transgenic plants for rice SEP-ike genes

  13. Phenotypes of wild-type and OsM7/8-RNAi spikelets OsM7/8 -RNAi WT WT OsM7/8-RNAi OsM7/8 -RNAi

  14. Scanning electron microscopyof epidermal cells expression patterns detailed structure WT 藉由這樣的實驗作者是要看小穗在OsM7/8-RNAi和WT當中，表皮細胞細部構造的表現情形 OsM7/8-RNAi OsM7/8 -RNAi

  15. Phenotypes of the OsM1/5/7/8-RNAi inflorescences and spikelets OsM1/5/7/8-RNAi WT OsM1/5/7/8-RNAi 9 6

  16. Under the Scanning electron microscopy the different developmental stages, formation of inflorescence meristem changes OsM1/5/7/8-RNAi 藉由這樣的實驗作者是要看小穗在OsM1/5/7/8-RNAi和在花序中產生變化所表現的情形

  17. Characteristics of the florets in transgenic lines palea(內穎) lemma(外穎) carpel(心皮) lodicule(漿片) stamen(雄蕊) 此表格作者所要呈現的是轉殖株在花部位的特性

  18. Expression patterns of OsMADS7 and OsMADS8 during inflorescence and spikelet development as revealed by in situ hybridization OsMADS8 OsMADS7 OsMADS7 OsMADS8 pbm代表主要分支 sbm代表次要分支 smc代表分生組織細胞;tsn代表末端分生組織;lsm代表橫向分生組織

  19. Meristem at different times for each organ, in part on the show are different OsMADS8 OsMADS7 藉由這樣的實驗作者是要看OsMADS7.OsMADS8在花序與小穗中分生組織表現的情形 OsMADS7 OsMADS8 fm代表分生組織

  20. Yeast two-hybrid assays. Interaction patterns of the rice SEP-like proteins including OsMADS1, OsMADS5, OsMADS7 and OsMADS8 are shown 藉由這樣的實驗作者證實了OsMADS1.OsMADS7.OsMADS8蛋白彼此之間的結合

  21. Interaction analyses of four rice class E proteins in vivo 藉由這樣的實驗作者證實了水稻中E class蛋白彼此之間的結合能力

  22. conclusion • Silencing of both OsMADS7 and OsMADS8 causes severe morphological alterations of floral organs. • Simultaneous silencing of OsMADS1, OsMADS5, OsMADS7 and OsMADS8 transforms all floral organs into leaf-like structures. • OsMADS1, OsMADS7 and OsMADS8 can cross-interact to each other while OsMADS5 can not.

  23. Thanks for your listening

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