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Expression Patterns and Localization of BZS1 in Transgenic Plants Under BR Treatment

Explore expression patterns and sub-localization of BZS1 using GUS reporter gene assays in transgenic plants treated with BR. The study includes dark and light conditions, adult leaf, flowering bud, flowers, and siliques examination.

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Expression Patterns and Localization of BZS1 in Transgenic Plants Under BR Treatment

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  1. A C A B B C D D B C A D 10nMeBR E F G 100nMeBR 100nMeBR F E G H H I J K Figure S2. Expression pattern and sub-localization of BZS1. (A-H) GUS reporter gene assays of BZS1::GUS transgenic plants in 3d seedlings grown in the dark without BR(A), 100nM eBR treatment in the dark for 3 days (B), 3d-grown seedlings in the light(C), 100nM eBR treatment in the light for 3 days (D), the adult leaf(E), flowering bud(F), flowers(G) and siliques (H). More than 5 independent transgenic wild type plants transformed with pBZS1::GUS were stained. Two lines were used in the BR treatment. (I-K) YFP fluorescence images of transgenic Arbidopsis plants expressing 35S:: BZS1-YFP construct.

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