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Contents of Bioseparation. Prof. In Ho Kim Chungnam National University. Chap.1 Introduction. Classification of Bioproducts Small molecules Macromolecules Proteins Nucleic acids and nucleotide Polysaccharides Engineering analysis. Chap.2 Analytical methods. Biological activity

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contents of bioseparation

Contents of Bioseparation

Prof. In Ho Kim

Chungnam National University

chap 1 introduction
Chap.1 Introduction
  • Classification of Bioproducts
  • Small molecules
  • Macromolecules
    • Proteins
    • Nucleic acids and nucleotide
    • Polysaccharides
  • Engineering analysis
chap 2 analytical methods
Chap.2 Analytical methods
  • Biological activity
  • Analysis of purity
    • Electrophoresis
    • HPLC
    • HPLC-Mass
    • Spectrophotometric assay
  • Microbiological assays
chap 3 cell lysis and flocculation
Chap.3 Cell lysis and Flocculation
  • Cell structure
    • Prokaryotic cells
    • Eukaryotic cells
  • Cell lysis
    • Chemical and mechanical methods
  • Flocculation
    • Electric double layer
    • Flocculation rate
chap 4 filtration
Chap.4 Filtration
  • Filtration principles
    • Conventional and crossflow filtration
  • Filter media and equipment
  • Membrane fouling
  • Scaleup and design of filtration system
chap 5 sedimentation
Chap.5 Sedimentation
  • Equation of motion
  • Equilibrium sedimentation
  • Sedimentation coefficient
  • Equivalent time
  • Production centrifuge
  • Ultracentrifugation
  • Sedimentation at low acceleration
chap 6 extraction
Chap.6 Extraction
  • Partitioning equilibria
  • Phase separation
  • Countercurrent stage calculation
  • Scaleup and design of extractors
chap 7 chromatography
Chap.7 Chromatography
  • Adsortion equilibrium
  • Column dynamics
    • Plate model
    • Rate model
  • Adsorbent types
  • Equipment
  • Scaleup
chap 8 precipitation
Chap.8 Precipitation
  • Protein solubility
  • Pricipitate formation phenomena
    • Nucleation, growth by diffusion and fluid motion, precipitate breakage and aging
  • Particle size distribution
  • Methods of precipitation
  • Design of precipitation system
chap 9 crystallization
Chap.9 Crystallization
  • Principles
    • Nucleation
    • Crystal growth
    • Kinetics
  • Batch crystallizers
  • Process crystallizers of proteins
  • Scaleup and design
chap 10 drying
Chap.10 Drying
  • Principles
    • Water in biological solids
    • Heat and mass transfer
  • Dryer description and operation
    • Vacuum shelf and rotary dryer
    • Freeze dryer
    • Spray dryer
  • Scaleup and design
chap 11 bioprocess design
Chap.11 Bioprocess design
  • Synthesis of bioseparation process
    • Primary recovery and final purification
  • Process analysis
    • Spreadsheet and simulators
  • Process economics
    • Capital and operating cost analysis
  • Examples(Insulin and citric acid production)