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An unnatural base pair for incorporating amino acid analogs into proteins

An unnatural base pair for incorporating amino acid analogs into proteins. Ichiro Hirao et al. Nature biotechnology, 20(2) 177-182. MEC Seminar Su Dong Kim 2003. 7. 1. Unnatural base pair of s-y expands the genetic code. s : 2-amino-6- (2-thienyl) purine y : pyridin-2-one

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An unnatural base pair for incorporating amino acid analogs into proteins

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  1. An unnatural base pair for incorporating amino acid analogs into proteins Ichiro Hirao et al. Nature biotechnology, 20(2) 177-182 MEC Seminar Su Dong Kim 2003. 7. 1.

  2. Unnatural base pair of s-yexpands the genetic code s : 2-amino-6- (2-thienyl) purine y : pyridin-2-one yAG codon • ClTyr : Chlorotyrosine ras protein

  3. Fig. 1 The unnatural x-y (A) and s-y (C), and noncognate x-T (B) and s-T (D) base pairs

  4. Fig. 2 T7 transcription employing s-y pairing

  5. Fig. 3 The transcription-translation system involving the s-y base pair

  6. Fig. 4 Preparation of aminoacylated tRNACUs

  7. Fig. 5a Autoradiogram of in vitro transcription-translation products labeled with I-[14C] leucine

  8. Fig. 5b LC patterns of the products digested by Lys-C

  9. Fig. 5c Tandem mass spectrum of F-cY32 fragment

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