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Supplemetal Fig. 1 - PowerPoint PPT Presentation


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LKB1. -. +. pAMPK(T172). HA-AMPK. FLAG-LKB1. LKB1. -. +. pAMPK(T172). AMPK. pACC(S79). FLAG-LKB1. Supplemetal Fig. 1. B. A. C. LKB1iSP. -. +. -. -. +. -. GFPRNAi. -. -. +. -. -. +. 2DG (20 mM). -. -. -. +. +. +. pAMPK(T172). AMPK. LKB1. Supplemetal Fig. 2.

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Presentation Transcript
slide1

LKB1

-

+

pAMPK(T172)

HA-AMPK

FLAG-LKB1

LKB1

-

+

pAMPK(T172)

AMPK

pACC(S79)

FLAG-LKB1

Supplemetal Fig. 1

B

A

C

LKB1iSP

-

+

-

-

+

-

GFPRNAi

-

-

+

-

-

+

2DG (20 mM)

-

-

-

+

+

+

pAMPK(T172)

AMPK

LKB1

slide2

Supplemetal Fig. 2

A

LKB1 -D194A

LKB1 -WT

AMPKi (M)

-

-

-

autorad

FLAG-LKB1

B

AMPKi (M)

-

+

-

+

-

-

+

+

LKB1

autorad

FLAG-LKB1

C

AMPK-DN

AMPK-WT

AMPKi (M)

-

-

autorad

Coomassie/

TSC2 fragment

HA-AMPK

slide3

Supplemetal Fig. 3

A

LKB1

-

+

Rapamycin (nM)

0

20

40

pS6K(T389)

pS6K(T389)

S6K

S6K

HA-LKB1

B

LKB1

-

-

+

+

-

-

+

+

-

+

+

+

Rheb

-

WT

-

WT

-

L64

-

L64

-

-

WT

5A

pS6K1(T389)

HA-S6K

FLAG-LKB1

Myc-Rheb