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DNA Fingerprinting & Gel Electrophoresis

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DNA Fingerprinting & Gel Electrophoresis. Do Now:. DNA Fingerprinting. Everyone (except identical twins) has a unique DNA sequence in their cells. A technique called \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_can be used to \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_(or parents) using their DNA.

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dna fingerprinting
DNA Fingerprinting
  • Everyone (except identical twins) has a unique DNA sequence in their cells.
  • A technique called ________________________can be used to ________________________(or parents) using their DNA.
  • DNA fingerprinting is MUCH more accurate than traditional fingerprints
how dna fingerprinting works
How DNA Fingerprinting Works
  • DNA is extracted from a sample (like when you got DNA from strawberries)
  • Special enzymes called _________________________ are used to cut the DNA samples into pieces.
  • The DNA pieces are then put through a process called _____________________________
gel electrophoresis
Gel electrophoresis
  • Gel electrophoresis is used to __________________________________.
  • DNA molecules are separated by ____________.
analyzing the results
Analyzing the Results

Restriction

Enzymes

  • The gel shown shows what happened when the same piece of DNA was cut up with 3 different enzymes.

DNA

fragment

Standard

Sizes

solving crimes rape investigation
Solving Crimes: Rape investigation
  • The DNA from a rapist can be collected from the victim for up to about 24 hours after the crime.
  • The DNA found can then be compared to the DNA of suspects.
  • Which suspect committed the crime?
dna fingerprint paternity testing
DNA Fingerprint Paternity Testing
  • DNA fingerprinting (with gel electrophoresis) can be used to determine who the __________ of an organism are.
  • Just like genes, DNA fragments are _____________ from both parents
final thoughts
Final Thoughts
  • A DNA fingerprint is like a unique _______________ for every organism on Earth.
  • DNA fingerprinting can be used to __________________________and determine who an individual’s genetic __________________ are.
  • DNA fingerprinting uses ________________________to separate different size pieces of DNA created by chopping up the original piece using special enzymes
mrna processing
mRNA Processing
  • Remember the central dogma of molecular biology:
    • ___________________________
  • In __________________, there is one more little step in between RNA and protein.
  • After an mRNA is made by transcription, it must be __________________ before _________________.
introns exons
Introns & Exons
  • An mRNA sequence has ___________ and __________ within it.
  • Before being used to make proteins, the introns must be ___________________.
  • Intron: A part of an mRNA molecule that ____________________________ before translation.
  • Exon: A part of an mRNA molecule that will be _______________________________.
get rid of those introns
Get rid of those Introns!
  • Before introns are removed, an RNA molecule is called ________________________________________
  • Only the ______________ make it to translation!
that s it for molecular genetics
That’s it for Molecular Genetics
  • Your review guide is due tomorrow!
  • We will be playing Jeopardy tomorrow: your review guide will be the only notes you may use.
  • Use the remainder of the period (and tonight) to complete anything on the review guide you haven’t yet.
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