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Li and Xue, Supplemental Figure 2. A. 0 1 nM 10 nM 100 nM 1 µ M (IAA). C LO11 LO19. C LO11 LO19. C LO11 LO19. C LO11 LO19. C LO11 LO19. pO-PLD z 2. 0 1 nM 10 nM 100 nM 1 µ M (IAA).

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Li and Xue, Supplemental Figure 2

A

0 1 nM 10 nM 100 nM 1 µM (IAA)

C LO11 LO19

C LO11 LO19

C LO11 LO19

C LO11 LO19

C LO11 LO19

pO-PLDz2

0 1 nM 10 nM 100 nM 1 µM (IAA)

C LA11 LA19

C LA11 LA19

C LA11 LA19

C LA11 LA19

C LA11 LA19

pA-PLDz2

B

Control

*

pO-PLDz2-LO11

45

*

*

pO-PLDz2-LO19

*

40

*

pA-PLDz2-LA3

*

35

pA-PLDz2-LA11

*

*

30

*

25

Primary root length (mm)

*

20

*

*

15

10

5

0

1 µM

100 nM

0

1 nM

10 nM

IAA concentration

  • Supplemental Figure 2. Altered Expression of PLDz2 Result in Altered Auxin Effects on Primary Root Growth.
  • Compared with controls (C), PLDz2-overexpressing plants were hypersensitive to auxin treatment (upper panel), whereas those deficient in PLDz2 were less sensitive (lower panel). Nine-day-old plants were employed for experiments. Arrows highlight differences in primary root length. Bar=1 cm.
  • Measurement of primary root lengths of control and transgenic plants with altered expressions of PLDζ2 grown on medium supplemented with various concentrations of IAA for 9 days confirmed hypersensitivity of PLDz2-overexpressing plants to auxin and relative insensitivity of PLDz2-deficient plants. Error bars indicate SE (n>40) and statistical analysis was performed using a one-tailed Student’s t test (*, P<0.01).