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Due Today: 1. Iron Content Analysis Pre-Lab 2. Neutralizing Power of Antacids Formal Lab Report Homework: 1. Iron Content Analysis Short Report 2. Prepare for Laboratory Final. Iron Content Analysis. Objective. Introduction to ultraviolet-visible (UV-Vis) spectroscopy

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iron content analysis

Due Today:

1. Iron Content Analysis Pre-Lab

2. Neutralizing Power of Antacids Formal Lab Report

Homework:

1. Iron Content Analysis Short Report

2. Prepare for Laboratory Final

Iron Content Analysis
objective
Objective
  • Introduction to ultraviolet-visible

(UV-Vis) spectroscopy

  • Introduction to analytical chemistry
  • Determine the iron content in vitamin tablets
key terms
Key Terms

Visible Range: 700-400 nm

UV Range: 10-400 nm

key terms1
Key Terms
  • Spectroscopy – measuring the interaction of radiation with matter
    • Unique wavelength for each substance
    • Scanning – determine wavelength with maximum absorption
    • Chemists are most interested in the electromagnetic spectrum between 190-900 nm
key terms2
Key Terms
  • Beer-Lambert Law - Absorbance of a solution is linearly related to concentration and identity of the chemical in solution
  • A: absorbance
  • b: extinction coefficient
  • c: concentration
procedure notes
Procedure Notes
  • Use Volumetric Glassware
    • Volumetric flask
    • Graduated pipets
    • Burets
  • Use stock solutions dispensed from burets
procedure notes1
Procedure Notes
  • Wipe off the cuvette with a Kim-Wipe before inserting into the Spectrophotometer
  • Record the absorbances!
  • Record the advertised Iron content from both vitamin bottles!
waste
Waste
  • Solutions can be disposed of down the drain
risk assessment
Risk assessment
  • Hydrochloric Acid
    • Danger
    • Inhalation Hazard
    • Corrosive
risk assessment1
Risk assessment
  • Hydroquinone
    • Danger
    • Harmful if Swallowed
    • Skin Irritant
risk assessment2
Risk assessment
  • o-phenanthroline
    • Danger
    • Toxic if swallowed
calculations
calculations
  • Molarity units: mg of Fe/100 mL
    • [Stock solution]= 40 mg/L=4 mg/100mL
  • V1= volume of stock iron solution in each trial
  • V2=100 mL (total volume)
calculations1
calculations
  • Graphing in Excel
    • Force the trendline through (0,0)
    • Include the equation of the tendline and R2 value

Y = mx

A = bc