Liquid Chromatography - PowerPoint PPT Presentation

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liquid chromatography n.
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Liquid Chromatography

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  1. Liquid Chromatography Chapter 28

  2. - Some of these can be used as hyphenated techniques

  3. Solute interactions in Reverse Phase Liquid Chromatography

  4. Separates based on size Large particles flow past packing w/o slowing Small particles spend time in pores of packing causing slower elution Size Exclusion Chromatography

  5. Exclusion limit – max MW of species that will penetrate pores -above this, all species elute as one pk Permeation limit – min MW of species that will penetrate pores completely -below this, all species elute as one pk

  6. Separation of Fatty Acids using Size Exclusion Chromatoragraphy