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Version Space 의 실험적 고찰

Version Space 의 실험적 고찰. 2001.11.23 장해만. Introduction. DNA structure Hybirdization time version space Other methods. DNA structure. biochemistry 4 th edition p788-793. Hybirdization time. biochemistry 4 th edition p990. Tm value 의 측정 ds strand : 40% less than ss at 260nm

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Version Space 의 실험적 고찰

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  1. Version Space의 실험적 고찰 2001.11.23 장해만

  2. Introduction • DNA structure • Hybirdization time • version space • Other methods

  3. DNA structure biochemistry 4th edition p788-793

  4. Hybirdization time biochemistry 4th edition p990 • Tm value의 측정 ds strand : 40% less than ss at 260nm • Kinetic of reassociation S : single strand S`: 상보적 seq. D : ds seq. K : 평형상수 Co: 전체DNA농도 f : ss decreases

  5. Hybridization time

  6. Hybridization time Low con. of complementary seq slow rate of reassociation Cot0.5 is directly related to the size of genome ex) mammalian genome(30억bp) ====104 Ms 10-4m sol. of DNA ===== 108 Ms (about 3 years) 10000도시 TSP : 모든 도시를 DNA로 표현 = 47 =16384; 적어도 7bp가 소요.. 7 * 10000 (weight제외) + weight(10000!) + ,,,

  7. cs ee four faculty staff five Version space Edge • Graph for All Hypothesis Vertex + Dummy strand for blunt end?

  8. Version Space • available method 1 • vertex 와 edge(PNA)를 이용, 가능한 모든 pool 합성 • positive seq. 제작 • hybridization in pool • PAGE로 위치확인 • gel elution • PCR , 적정농도의 pool 형성 ---1st generalize • 2~6 반복 ( 단, positive일 경우) • negative example ??? • DNA breaks down in gel elution

  9. Version Space • available method 2 • 가능한 모든 pool을 주문 • positive seq. 제작 (linked bead) • hybridization in pool • bead selection • enz.처리 ---- blunt end 형성 • denaturation --- 1st generalize pool • negative seq. 제작(linked bead) • pool을 일부 균등분할 / hybridize / selection / enz. cutting / PAGE • PAGE에서 나온 band sequencing • negative probe (linked bead) 제작 • 1st generalize pool과 hybrid / bead selection / discard • 농도차이에 의한 부정확한 구분?? • 비용 / 비능률

  10. Other Methods • Megaclone • Megasort • Megatype • Mpss

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