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Real World Surveillance, LLC. 22 nd Annual Produced Water Society Seminar January 17, 18, 19 th , 2012. Microbial Management to Improve OBW Discharge, H2O Injection and H2O Reuse. Real World Surveillance, LLC Telephone: 601.250.6256
22nd Annual Produced Water Society Seminar
January 17, 18, 19th, 2012
Microbial Management to Improve OBW Discharge, H2O Injection and H2O Reuse
Real World Surveillance, LLC Telephone: 601.250.6256
Johnny D. Richard, Owner/Operator Cell: 601.551.0395
Certified Minority/Veteran Business Enterprise Email: firstname.lastname@example.org
Website: www.realworldeye.com Email: email@example.com
“A Hands On, Eyes Open Company”
“A Real Eye in the Real World”
Sessile -- Biomass
Planktonic -- Floaters
If You Don’t Measure It, You Can’t Control It!
The cellular DNA of microorganisms like bacteria absorbs the energy from the UV light, causing damage to their DNA structure this damage interferes with many cellular processes including protein synthesis then the replication of the chromosome prior to reproduction is impaired, leaving the bacteria unable to produce proteins or replicate.
The Eco1st Separation Enhancer "separates oil & water" by causing the water molecules to lose their ability to bond with one another and any foreign contaminates contained therein. The Separation Enhancer does this by breaking dipole bonds and stripping electrons from the water and the foreign contaminates which ionizes the water into a positively charged state. When ionized the molecules repel one another and make it easy for separation of these materials to occur.
Reduce need for chemicals and biocides. *Information pulled directly from ECO1ST website.
Traditional Test Methods:
Serial Dilution & Biological Activity Reaction Test (BART):
Note: If our goal is to improve OBW quality, then we should use a method that fits that purpose.
Real World Test Methods for:
Water, Fuel, Oily-based-fluid, Deposits and Surface
Project Description - Example
Testing Protocol Baseline:
*Note: Any chemical suspected of having an effect on the microbial levels of the produced water should be adjusted or optimize prior to start. Measure Wemco 02 levels
Testing Protocol Baseline cont’d
Optional Baseline Data:
Disposal Well (if applicable)
Ensure all equipment are in good working order: Chemical injection system, O&G batch/online monitor, good supply of sample bottles, S.O. machine, etc.
Primary Contamination Sources: Formation, Vessels(Bottoms/Interface/Fluid Outlets), Wet/Bad Oil Tanks, Treaters, & Production Sumps
For heavily contaminated systems, e.g. high ATP values >100,000 pg/mL in all locations, you might want to see how effective is your biocide batch method first, after one week or one day. In such cases a continuous treatment maybe in order to establish some control. Continuous treatment rate can be controlled using the ATP method.
On the other hand, if there are minimum oil and water quality issues on your platform, you may have bacteria species that are not affecting the process.
Auxiliary WHRU/Steam systems that do not contain ethylene glycol should be checked also due the reality, if you lose your heat medium system, oil and water separation becomes more of a challenge. The same can be said for the drinking water and/or potable water system. Sick personnel means not much gets done.
Testing Protocol Biocide Treatment:
Testing Protocol Biocide Treatment cont’d:
Note: GR = EPA 1664 HEM, GRS = EPA SGT-HEM
Modified Testing Protocol: Baseline and Protocol Testing
Modified Biocide Testing: 1) Test H2O outlet of vessel prior to biocide treatment, 2) Test H2O after treatment: Frequency: Day 1 (Hour 1, 4, 8, 16 & 24), then daily……
Verdict: All methods are unique!
Viable sub-populations of total populations
Estimate of activity
of total population
Microbial Management Programs
What is Your Microbial Management Plan to Improve
OBW Discharge, H2O Injection and H2O Reuse?
2nd Generation ATP Technology
“The First Line of Defense Tool”
LuminUltra 2nd Generation ATP Test Methods
With field test results.
BART’s – Biological Activity Reaction Testers
ASTM Approves Method for ATP Measurement in Fuels
Principal Microbial Groups Determinable Using the BART™ Biodetectors
Microbial Community Selective Culture Medium*
Iron Related Bacteria IRB Winogradsky’s medium
Sulfate Reducing Bacteria RB Postgate’s medium
Slime Forming Bacteria SLYM Glucose Peptone medium
Heterotrophic Aerobic Bacteria HAB Sugar Peptone medium
Algae ALGE Bold’s medium
Fluorescent Pseudomonads FLOR Peptone base medium
Denitrifying Bacteria DN Nitrate Peptone medium
Nitrifying Bacteria N Ammonium salts medium
Acid Producing Bacteria APB Modified Glucose Peptone medium
Biochemical Oxygen Demand BOD Modified Sugar Peptone medium