Structure and composition of nuclear pore complexes npcs
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Structure and composition of nuclear pore complexes (NPCs). Electron micrographs of NPCs. Gall, 1967. Electron micrographs of NPCs. Gall, 1967. Three-dimensional view of a nuclear pore in the double-layered nuclear envelope. Gall, 1967.

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Structure and composition of nuclear pore complexes npcs

Three-dimensional view of a nuclear pore in the double-layered nuclear envelope

Gall, 1967


Structure and composition of nuclear pore complexes npcs

Diagram of a model cylinder with the x-, y- and z-axes double-layered nuclear envelope

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

Averaged xy slices taken from the 3D map of the NPC double-layered nuclear envelope

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

3D side view of the NPC surface map double-layered nuclear envelope

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

z slices taken from 3D maps of the NPCs double-layered nuclear envelope

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

Surface views of the NPC 3D map double-layered nuclear envelope

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

3D representation of the membrane-associated NPC double-layered nuclear envelope

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

3D structure of the NPC double-layered nuclear envelope

Beck et al, 2004


Structure and composition of nuclear pore complexes npcs

Cutaway view of the NPC double-layered nuclear envelope

Beck et al, 2004


Structure and composition of nuclear pore complexes npcs

Slices through the structure of the pore double-layered nuclear envelope

Beck et al, 2004


Structure and composition of nuclear pore complexes npcs

The NPC nuclear basket double-layered nuclear envelope

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

The NPC cytoplasmic particles double-layered nuclear envelope

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

The NPC cytoplasmic particles double-layered nuclear envelope

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

Immuno-labelling of Nup116 double-layered nuclear envelope

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

Summary double-layered nuclear envelope

The NPC has eight rotational symmetry.

The NPC is constructed from three main parts: 1) cytoplasmic components 2) central parts3) nucleoplasmic components

The NPC is huge structure. The vertebrate NPC has about 125 nm diameter (around 20 times larger than a ribosome).


Structure and composition of nuclear pore complexes npcs

Comparison of the peripheral and intrinsic membrane polypeptides of nuclear envelopes and microsomal membranes

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Characterization of antibodies to gp 190 and the lamins polypeptides of nuclear envelopes and microsomal membranes

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Immunofluorescence staining of tissue culture cells with anti-gp 190 and anti-lamins A and C

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Immuno-labeling of nuclei with anti-gp 190 anti-gp 190 and anti-lamins A and C

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Presence of gp 190 in diverse vertebrate species anti-gp 190 and anti-lamins A and C

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Mammalian, yeast and worm nucleoporins anti-gp 190 and anti-lamins A and C

Angelo & Hetzer, 2008 (Review)


Structure and composition of nuclear pore complexes npcs

Summary anti-gp 190 and anti-lamins A and C

The NPC is composed of ONLY about 30 different proteins (nucleoporins). Ribosome has 80 different protein components.

Like structure, the composition of NPC is highly conserved between human and yeast.


Structure and composition of nuclear pore complexes npcs

Schematic illustration of the NPC structure anti-gp 190 and anti-lamins A and C

Angelo & Hetzer, 2008 (Review)


Predicted composition of scaffold and central channel
Predicted composition of scaffold and central channel anti-gp 190 and anti-lamins A and C

Angelo & Hetzer, 2008 (Review)


Predicted composition of cytoplasmic components
Predicted composition of cytoplasmic components anti-gp 190 and anti-lamins A and C

Angelo & Hetzer, 2008 (Review)


Predicted composition of nuclear components
Predicted composition of nuclear components anti-gp 190 and anti-lamins A and C

Angelo & Hetzer, 2008 (Review)


Predicted composition of transmembrane components
Predicted composition of transmembrane components anti-gp 190 and anti-lamins A and C

Angelo & Hetzer, 2008 (Review)


Predicted localization of sub complexes and nucleoporins within the npc
Predicted localization of sub-complexes and nucleoporins within the NPC

Angelo & Hetzer, 2008 (Review)


Structure and composition of nuclear pore complexes npcs

Summary within the NPC

The nuclear pore complex in turn is made of several protein sub-complexes.

The NPC sub-complexes are divided to two categories:1) peripheral 2) central