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TISSUE BIOREPOSITORY AND PATHOLOGY SERVICES

TISSUE BIOREPOSITORY AND PATHOLOGY SERVICES. TISSUE PROCUREMENT. Routinely collect tumor tissue, adjacent normal tissue, buffy coat, plasma, newly diagnosed and pre-transplant hematologic samples Also collect urine, saliva, and biopsies for particular projects

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TISSUE BIOREPOSITORY AND PATHOLOGY SERVICES

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  1. TISSUE BIOREPOSITORY AND PATHOLOGY SERVICES

  2. TISSUE PROCUREMENT • Routinely collect tumor tissue, adjacent normal tissue, buffy coat, plasma, newly diagnosed and pre-transplant hematologic samples • Also collect urine, saliva, and biopsies for particular projects • All tissue samples receive quality control analysis before distribution • Site specific committees comprised of surgeons and researchers must approve request for samples that are in low inventory. Ensures that valued samples are given to important projects

  3. DONOR EVENTS 2011*event could be any combination of tissue and blood samples

  4. Collections: 2010 vs. 2011 Each month, we have increased our tissue collection over what it was in the previous year

  5. Pathology Services • Tissue fixation, processing, and embedding • Frozen and FFPE tissue sectioning • H&E staining • Immunohistochemistry • RNA isolation and BioAnalyzer • LCM • Tissue MicrroArray • *In the process of offering special stains * • Other requests …

  6. Laser Capture Microdissection Cryostat & Microtome PixCell II XT Bioanalyzer RiboAmp TM & Paradise TM Cell Selection Extraction & QC Amplification Amplified Unamplified H&E section Extracted RNA qPCR analysis Captured cells After capture

  7. BIOANALYZER • Determine sample quality before performing downstream applications • Faster than gels – up to 12 samples in 30 min • Minimal sample consumption; 1 μL • Ready-to-use reagent kits • Chip preparation in less than 5 min

  8. TISSUE MICRO ARRAY • Services provided: • Custom TMA construction • TMA slide sectioning • Immunohistochemistry and in situ hybridization on TMA slides • TMA pathology imaging scoring • Support for grant applications by providing LCM budgets and support letters

  9. Contact InformationKiki GibbsPhone: 843-792-8165E-mail: gibbski@musc.eduLocation: room 421 Bioengineering Building

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