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Protein synthesis - PowerPoint PPT Presentation


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Protein synthesis. Unusual bases in tRNA. “Wobble” hypothesis. Functional domains of a ribosome. Charging of tRNA/Aminoacylation. Translation - 4 main steps Charging of tRNA Initiation Elongation (3 steps) Termination. Initiation in Prokaryotes. Elongation in Prokaryotes.

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Presentation Transcript
slide18

Translation - 4 main steps

Charging of tRNA

Initiation

Elongation (3 steps)

Termination

proofreading on the ribosome
Proofreading on the ribosome

The GTPase activity of EF-Tu .

Polyribosomes(or polysomes)

slide31

POST TRANSLATIONAL CHANGES

Dealing with N terminal residue

Modification of individual amino acids

Acetylation

Phosphorylation

life span factors
Life-span factors
  • Natural stability ("genetically encoded")
    • an inherent biophysical characteristic
  • Change in environment
    • temperature
    • pH
  • Active degradation
    • specific mechanism
    • location
    • partners
terminology
Terminology
  • Half-life -
  • Turnover -
  • Stability -
  • Denaturation -
  • Degradation -