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Enzymes - PowerPoint PPT Presentation


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Enzymes. The Drunk Man that Never Drank. Candida albicans – Mutation to a fermenting form. Microbial metabolism includes fermentation Metabolism definition Anabolic vs catabolic Both require enzymes. Micro Focus 5.1. Origin of the term enzyme – in yeast

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Presentation Transcript
the drunk man that never drank
The Drunk Man that Never Drank
  • Candida albicans –
  • Mutation to a fermenting
  • form
slide3
Microbial metabolism includes fermentation
  • Metabolism definition
  • Anabolic vs catabolic
  • Both require enzymes
micro focus 5 1
Micro Focus 5.1
  • Origin of the term enzyme – in yeast
  • Discovery by the Buchner brothers
slide6
1. Enzymes

Definition

A reusable protein molecule that brings about chemical change while remaining unchanged itself.

slide7
2. General Characterisitics

Highly Specific

Sensitive to the environment

May function in enzyme systems or pathways

Enzyme names end in -ase

slide9

Apoenzyme

    • Large molecular wt.
    • All protein
    • Contains active site

Holoenzyme

+

=

  • Coenzyme
      • Small molecular wt
      • Organic

or

  • Cofactor
      • Inorganic
      • Metallic
slide11
2. Mechanism of activity

Active Site

Substrate

Products

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3. Enzyme Classification

Based on where the enzyme activity occurs.

Exoenzymes

Endoenzymes

slide15
3a. Enzyme Classification

Based on type of enzyme function

Oxidoreductase

O/R reactions

Electron transfer

Transferase

Moves organic functional groups

Example: deaminase

slide16
3b. Enzyme Classification

Based on type of enzyme function

Hydrolases

Hydrolysis reaction

H and OH ions

Bacterial enzymes

Coagulase

Fibrin formation

Streptokinase

Dissolves fibrin clots

slide18
1. Factors

Temperature

slide20
3. Factors

Substrate Concentration

slide22
1.Types of Inhibition

Competitive

Folic Acid inhibition by Sulfanilamide competition with PABA

Noncompetitive

Fluoride binding with Ca or Mg atoms in enzymes