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Proteins: Structure, Chemical Identification & Polypeptide Synthesis. Using Biuret to Identify Presence of a Protein Learning the Chemical Structure of an Amino Acid Using a Dehydration Synthesis to Make a Polypeptide Chaine. Testing for Organic Compounds Part A: Protein Testing.

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Presentation Transcript
proteins structure chemical identification polypeptide synthesis

Proteins: Structure, Chemical Identification & Polypeptide Synthesis

Using Biuret to Identify Presence of a Protein

Learning the Chemical Structure of an Amino Acid

Using a Dehydration Synthesis to Make a Polypeptide Chaine

testing for organic compounds part a protein testing

Testing for Organic CompoundsPart A: Protein Testing

1. Involves an indicator, Biruet Solution

2. Biuret reacts with the amino acids in the protein

3. Solution color goes from blue to violet

4. Color change indicates a chemical reaction between the biuret and the bonds between the amino acids of a protein

another look at biuret test
Another Look at Biuret Test

Postive Control

Experiment

Negative Control

Chemistry of the Biuret Test

proteins
Proteins
  • Are organic polymers composed of monomers called amino acids.
  • Strands of amino acids are called polypeptide chains. Where are the peptide bonds?

Proteins are many chained structures.

slide5

Polypeptide Chain

Amino Acids

Protein with two

Polypeptide chains

Where is the complete protein?

lab safety cleanup
Lab Safety & Cleanup
  • Test tubes are glass.
  • Biruet Solution is a base. Wash hands with water.
  • Goggles on at all times
  • Use special glassware soap and water with test tube brush to wash all test tube
  • Return all materials to appropriate location
  • Complete data table.
  • On back of lab, construct (5) observation statements and then write a conclusion.
polypeptide synthesis
Polypeptide Synthesis
  • Involves two amino acids.
  • Involves a dehydration synthesis.
  • Involves a chemical reaction that occurs between two specific areas of the amino acid.
  • Requires an –OH group and an –H from another –OH group
functions of proteins
Functions of Proteins

http://www.youtube.com/watch?v=iB7B0YaSLhU