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Camille Wolf, Annalisa Pavese, Urs von Gunten , Tamar Kohn

Inactivation of Waterborne Viruses by Ozone in Natural Matrices : Proxies for viral inactivation. Camille Wolf, Annalisa Pavese, Urs von Gunten , Tamar Kohn. Why does this matter ?. Quantitative Microbial Risk Analysis. Wastewater treatment. Guidelines or legislation.

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Camille Wolf, Annalisa Pavese, Urs von Gunten , Tamar Kohn

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  1. Inactivation of Waterborne Viruses by Ozone in Natural Matrices : Proxies for viral inactivation Camille Wolf, Annalisa Pavese, Urs von Gunten, Tamar Kohn

  2. Why does this matter ? Quantitative Microbial Risk Analysis Wastewater treatment Guidelines or legislation (Potable) Water Reuse Gerrity (2012) /Andrey Kekyalyaynen/ ELSEVIR/ http://www.greatlakestoday.org

  3. Challenges • Impossible to monitor infective virus • kO3-virus is unknown • Impossible to know exposure Ozone exposure Inactivation Inactivation rate constant

  4. Kinetics in buffer system • Simplified system • Ozone exposure is controlled by chemistry • kO3-Virus Temperature : 2, 12 and 22°C 9 viruses & bacteriophages Natural matrices Bacteriophages Human viruses

  5. Objectives ? Ozonation [Virus]i [Virus]f • Confirm O3 efficiency in natural matrices • Validate potential of proxies MS2 Coxsackievirus B5 Virus specific O3 dose ΔUV254 Carbamazepine Proxies [Proxy]f [Proxy]i d[Proxy] d[Virus] Humic acid Stevenson F.J. (1994). Humus Chemistry: Genesis, Composition, Reactions. New York: John Wiley & Sons.

  6. Methods: Natural matrices https://map.geo.admin.ch

  7. Methods: O3 exposure in natural matrix • Quenched flow Exposure vs specific O3 Dose Dose [gO3/gDOC] R script

  8. Methods: O3 exposure in natural matrix O3 Dose • Inactivation : MPN or Plaques Assay Exposures

  9. Results : kO3-virus kO3-virusBuffer system (Wolf et al., 2018)

  10. Methods : Proxies O3 Dose • Batch experiment • ΔUV254 • Inactivation : Cell culturing methods • CBZ : HPLC Laboratory Precision LimitedMatTek Corporation

  11. Results: Proxies vs O3 specific dose Specific O3 dose, [gO3/gDOC] Specific O3 dose, [gO3/gDOC]

  12. Results: Inactivation vs specific O3 dose

  13. Results: Inactivation vs ΔUV254

  14. Results: Inactivation vs CBZ abatement

  15. Predicted inactivation during WW treatment CBZ abatement ΔUV254 Data from Lester et al. (2012) extracted using webplotDigitilizer and recomputed

  16. Predicted inactivation during WW treatment ΔUV254Up to 5 log CBZUp to 2.5 log CBZ abatement ΔUV254 Prediction does not include the “Plateau”

  17. Conclusions • Specific O3 dose water specific • ΔUV254 is a good proxy for water with sufficient high [DOC] • CBZ abatement is a good proxy over wider range of [DOC]

  18. Thanks for your attention !Question ?!

  19. References Gerrity, D.; Gamage, S.; Jones, D.; Korshin, G. V.; Lee, Y.; Pisarenko, A.; Trenholm, R. A.; von Gunten, U.; Wert, E. C.; Snyder, S. A. Development of Surrogate Correlation Models to Predict Trace Organic Contaminant Oxidation and Microbial Inactivation during Ozonation. Water Res.2012, 46 (19), 6257–6272. Lester, Y.; Mamane, H.; Zucker, I.; Avisar, D. Treating Wastewater from a Pharmaceutical Formulation Facility by Biological Process and Ozone. Water Res.2013, 47 (13), 4349–4356. Gamage, S.; Gerrity, D.; Pisarenko, A. N.; Wert, E. C.; Snyder, S. A. Evaluation of Process Control Alternatives for the Inactivation of Escherichia Coli, MS2 Bacteriophage, and Bacillus Subtilis Spores during Wastewater Ozonation. Ozone Sci. Eng.2013, 35 (6), 501–513. Lee, Y.; Kovalova, L.; McArdell, C. S.; von Gunten, U. Prediction of Micropollutant Elimination during Ozonation of a Hospital Wastewater Effluent. Water Res.2014, 64, 134–148. Carvajal, G.; Branch, A.; Michel, P.; Sisson, S. A.; Roser, D. J.; Drewes, J. E.; Khan, S. J. Robust Evaluation of Performance Monitoring Options for Ozone Disinfection in Water Recycling Using Bayesian Analysis. Water Res.2017, 124, 605–617. Wolf, C.; von Gunten, U.; Kohn, T. Kinetics of Inactivation of Waterborne Enteric Viruses by Ozone. Environ. Sci. Technol.2018, 52 (4), 2170–2177.

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