Cutthroat trout in Colorado: A case study connecting evolution and conservation

1. Cutthroat trout in Colorado: A case study connecting evolution and conservation Sierra M. Love Stowell & Andrew P. Martin Student Figures

2. Figure 1: A phylogram of mitochondrial DNA sequence data from 15 cutthroat trout populations in Colorado and New Mexico. 10 unique Colorado River (blue) and Greenback (green) haplotypes were detected (A-J) from 1530 bp of the genes COI and ND2. Rio Grande haplotypes were collapsed into a monophyletic triangle (orange). Yellowstone and Lahontan cutthroat trout were used to root the tree (not shown). Statistical support is shown at major nodes. (Metcalf et al. 2007, with permission)

3. Figure 2: Statistical clustering of genetic variation for 10 microsatellite loci and 401 AFLP loci in the program STRUCTURE for individuals from 15 cutthroat trout populations in Colorado. Clustering asked how well each individual assigned to the “blue” or “green” groups. Microsatellites and AFLPs are nuclear genetic markers. Rio Grande populations are excluded. Probability of assignment to one of two clusters is shown along the x-axis. A horizontal line represents an individual; white lines designate populations. Thick white/black line indicates populations that are mostly “blue” type (above) or mostly “green” type (below). (Metcalf et al. 2007, with permission)

4. Figure 3: Cutthroat trout found in Colorado and the character states of morphological characters commonly used for trout identification (data from Behnke 1992; images: Wikimedia Commons).

5. Figure 4: Left, map showing a portion of inland western United States. Blue and green represent the presumed ancestral ranges of Colorado River and greenback cutthroat trout, respectively. Right, the portion within the rectangle is enlarged showing the approximate locations of 15 surveyed populations colored according to their subspecies designation. The thick black line running down the center of the figure indicates the Continental Divide and the labeled sub-basins on each slope are delineated with thinner black lines. The orange fish represents the native Rio Grande cutthroat trout, which is found in the Rio Grande drainage in southern Colorado and New Mexico. (Metcalf et al. 2007, with permission)

6. Figure 5: Top left, cumulative numbers in millions of trout stocked in Colorado from 1885 to 1953 by state and federal agencies. Dates of museum collections used in this study are indicated by arrowheads. Right a) Numbers of cutthroat trout stocked using divergent lineages from different drainage basins, colored by hypothesized origin of the lineage (Grand Mesa or Trapper’s Lake Hatcheries). Right b) Modern genetic identity of “pure” cutthroat trout populations in Colorado. (Metcalf et al. 2012, with permission)

7. Figure 6:A) Parsimony haplotype network from a subset of 430 bp of combined ND2 and COI mitochondrial sequence data, colored by identity. Haplotypes present only in museum samples have a black outline; haplotypes present in both modern and museum samples have a gray outline; haplotypes present only in modern samples have no outline. Open circles represent a putative mutation separating a haplotype from another. Numbers refer to fish sample IDs. B) Geographic distribution of museum samples, with the color of each circle corresponding to the lineage color in the network. The size of each circle is proportional to the number of museum samples within a particular lineage. (Metcalf et al. 2012, with permission)

8. Figure 7: Phylogenetic inference for the extant lineages of cutthroat trout in the Southern Rocky Mountains based on 430 bp on mitochondrial DNA sequence data. Samples include both modern and museum haplotypes. Statistical support values are shown for branches. Monophyletic clades of haplotypes are collapsed into triangles; the size of the triangle is proportional to the diversity present in the clade. Colored triangles represent those that were sampled in Colorado. (Metcalf et al. 2012, with permission)