Cell Cultuirng for Artifical Skin Report - IntellectPeritus

1. Sample Report on Novel Cell Culturing Techniques for Creating Artificial Skin At IntellectPeritus, we have collected around 50 patent documents and 25 Non-patents related to Novel Cell Culturing Techniques for Creating Artificial Skin as sample report and have tried to put value added technology categories to generate specific insights quickly using our deliverable InnovigenceCorona®. We created few insights based on the details of sample report. If you are interested in complete or any customized report for Indoor Air Sanitizer please feel free to write to us. All rights reserved. No part of this work may be produced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or inclusion in any information storage and retrieval system, without the prior written permission of IntellectPeritus Services.

2. Introduction Until the late twentieth century, skin grafts were constructed from the patient's own skin (autografts) or cadaver skin (allografts). Infection or, in the case of cadaver skin, rejection were primary concerns. While skin grafted from one part of a patient's body to another is immune to rejection, skin grafts from a donor to a recipient are rejected more aggressively than any other tissue graft or transplant. Artificial skin is collagen scaffold that induces regeneration of skin. An artificial skin offers several advantages over those derived from the patient and cadavers. It eliminates the need for tissue typing. Artificial skin can be made in large quantities and frozen for storage and shipping, making it available as needed. Each culture is screened for pathogens, severely curtailing the chance of infection. Because artificial skin does not contain immunogenic cells such as dendritic cells and capillary endothelial cells, it is not rejected by the body. Finally, rehabilitation time is significantly reduced. At IntellectPeritus, we have collected around 50 patent documents and 25 Non- patents related to Novel Cell Culturing Techniques for Creating Artificial Skin as sample report and have tried to put value added technology categories to generate specific insights quickly using our deliverable InnovigenceCorona®. If you are interested in detailed or any customized report for Novel Cell Culturing Techniques for Creating Artificial Skin, please feel free to write to us at sales@intellectperitus.com To schedule online meeting/demo please visit https://intellectperitus.com/request_demo.php

3. Technology Categories - Broad

4. Sample Patent Details 1. US20180087030A1 Title Artificial skin culture container and method for producing artificial skin using same Assignee Amorepacific Corp Publication Date 3/29/2018 Novelty: Culture container for artificial skin, which can prevent contraction of the dermis during the production of the artificial skin comprises agar in gel form, where a portion of the agar is hydrophobically modified. Mapped Technology:

5. 2. EP2543396B1 Title Artificial skin Assignee Shiseido Co Ltd Publication Date 4/10/2019 Novelty: Method for producing artificial skin in which communication between the epidermal basement membrane and dermis is adequately achieved and is as close as possible to the structure of natural skin comprising adding a matrix metalloproteinase inhibitor and a heparanase inhibitor to an artificial skin formation culture medium having human epidermal keratinocytes and human dermal fibroblasts and culturing the cells in the culture medium. Mapped Technology:

6. 3. WO2019221470A1 Collagen gel strength controller, method for manufacturing artificial skin by using same, and artificial skin Amorepacific Corp Title Assignee Publication Date 11/21/2019 Novelty: Method for preparing artificial skin which can be used as an aging research platform comprising preparing a dermal replica layer by mixing fibroblasts, collagen and the collagen gel strength modifier and placing the keratinocytes on the dermis replicating layer and culturing for two days. Mapped Technology:

7. 4. KR2016022470A Title Skin equivalent comprising avian collagen and the method preparing the same CHUNG ANG UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION Assignee Publication Date 3/2/2016 Novelty: Artificial skin for skin toxicity testing and medical transplantation comprises the use avian collagen as a component of the dermal substitute to induce proliferation of fibroblasts in the dermis and keratinocytes in the epidermis Mapped Technology:

8. 5. US8097274B2 Title Skin substitutes, preparation methods and uses thereof National Defense Medical Center Assignee Publication Date 1/17/2012 Novelty: Skin substitutes for use in a living subject for purpose of repairing damaged tissues comprising biocomposite membrane and at least one layer of cells grown on at least one side of the membrane, where membrane comprises poly(caprolactone) and at least one material selected from collagen and gelatin. Mapped Technology:

9. 6. KR2020031018A Title Epidermis and dermis matrix similar to the real skin and artificial skin comprising the same Assignee Korea Inst Sci & Tech Publication Date Novelty: Artificial skin having artificial epidermal layer and artificial dermis having physical properties similar to that of a biological skin, where artificial epidermal layer includes an electrospun porous biodegradable polymer, collagen coating layer coated on the biodegradable polymer, and a stratum corneum cultured with keratinocytes on the collagen coating layer Mapped Technology: 3/23/2020

10. Sample Scientific Literature Details 7. Article-1 Artificial skin - culturing of different skin cell lines for generating an artificial skin substitute on cross-weaved spider silk fibres Hanna Wendt, Anja Hillmer, Kerstin Reimers, Joern W Kuhbier, Franziska Schafer-Nolte, Christina Allmeling, Cornelia Kasper, Peter M Vogt Title Author Publication Date 2011-07-26 Novelty: Method for generating an artificial skin substitute comprising (1) Harvesting of spider dragline silk and manufacturing of weaving frames with cross-woven spider silk (2) Place frames with cross-woven spider silk into a well and place 100 ml cell suspension containing mouse embryonic fibroblasts (MEF) in MEF-specific medium onto middle of the frame, where MEF-specific medium contains Dulbecco's modified Eagle's medium (DMEM) High Glucose Cell culture medium supplemented with 10% fetal calf serum (FCS), 1% Sodium-pyruvate (PAA) and 1% Gentamycin solution (3) Place plates into an incubator for 30 minutes to promote cell attachment on the silk fibres, later fill up with 2 ml MEF-specific medium and culture for two weeks (4) On day 15, add HaCaTs (Human adult Calcium high temperature keratinocytes) on the confluent MEF layer, where before adding HaCaTs, polymer fibres were placed under the frame to achieve regular alignment of two cell types (5) Replace the medium in DMEM/Hams F12 in a 3:1 ratio supplemented with 1% FCS, 1% Sodium-pyruvate (PAA) and 1% Gentamycin solution (6) Start cultivation at the air liquid interface on day 16, and cultivate for additional 3 weeks, where media is changed three times a week Mapped Technology:

11. 8. Article-2 Cultured keratinocytes and dermal fibroblasts on a double-layer scaffold with bi-medium culture system Yi-Chau Huang, Tzu-Wei Wang, Jui-Sheng Sun, Feng-Huei Lin Title Author Publication Date 2003-01-01 Novelty: Development of skin equivalent comprising (1) Seed human dermal fibroblasts to form three-dimensional dermal replacement tissue (2) Initially culture keratinocytes in keratinocyte serum free medium supplied with epidermal growth factor (EGF) (3) After two days, change the medium to keratinocyte basal medium (without EGF) and subsequently culture for 14 days by the air-liquid interface culture method Mapped Technology:

12. 9. Article-3 Title Bioengineered self-assembled skin as an alternative to skin grafts Mihail Climov, Erika Medeiros, Evan A Farkash, Jizeng Qiao, Cecile F Rousseau, Shumin Dong, Agatha Zawadzka, Waldemar J Racki, Ahmad Al-Musa, David H Sachs, Mark A Randolph, Christene A Huang, Thomas J Bollenbach Author Publication Date 2016-06-10 Novelty: Generation of autologous bilayered living cellular construct (aBLCC) and autologous skin construct (ASC) comprising (1) Creating dermal fibroblast and keratinocyte cell banks from 6-mm skin punch biopsies of neonatal (0 to 3 days) MGH miniature swine and Yorkshire swine (2) Producing lower (dermal) layer of the ASC and aBLCC from dermal fibroblasts by seeded into a 75-mm diameter tissue culture inserts, where cells are cultured in Dulbecco's Modified Eagle's Medium supplemented only with 50 [mu]g/mL L-ascorbic acid-2-phosphate and 20 % fetal bovine serum (3) Generating upper (epidermal) layers of the ASC and aBLCC from keratinocytes by seeding onto dermal matrices (4) Lift tissue inserts to the air-liquid interface, with tissues receiving periodic media replenishment to promote epidermal stratification and barrier function development, which are induced to differentiate and stratify Mapped Technology:

13. 10. Article-4 A bilayer engineered skin substitute for wound repair in an irradiation-impeded healing model on rat AB Mohd Hilmi, Asma Hassan, Ahmad Sukari Halim Title Author Publication Date 2015-05-06 Novelty: Fabrication of engineered skin substitute comprising (1) Isolation of hair follicle stem cells (HFSCs) and Human Dermal Fibroblasts (HDF) (2) Seed 1 cm by 1 cm chitosan skin templates with HDFs (3) After 2 weeks, seed HDFs cultured in chitosan with HFSCs (4) Carry out HFSCs/HDFs co-culturing for 1 week using a combination of DMEM/F12 supplemented with 5 % FBS and CnT07 medium at a ratio of 1:2 (5) Harvest bilayer engineered skin substitutes after 1 week of coculture Mapped Technology:

14. Empowering Clients with strategic & customized “IP” solutions clubbed with industry leading Analytics Deliverable Platforms innovigenceCorona® & innovigenceCoronium® Landscape & Portfolio report includes insights representing various Patent Analytics Activities such as… Patent filing trend as over Timeline (Year) and geographical region (Including all family member patents & analyzed patents) Research Country Analysis (Invention Origin Country) and Competition/Marketing Country Analysis as geographical coverage over Time (filing) & Assignee Assignee Analysis as Top Assignees, Top Assignee filing trend, Filing Assignee/Organization type, Assignee - Law firm association all including Parent-subsidiary relationship criteria Inventor Analysis as Key Inventor(s) filing over geographical region & with Assignee(s) and Independent / Self Inventors Prosecution Analysis as Assignee Portfolio (Granted & Pending), Assignee Analysis Over Country Wise Granted Patents and Assignee Analysis Over Correspondent Patent Class Analysis as Cooperative Patent Classification (CPC) (Top over Timeline, Assignee & definition) and International Patent Classification (IPC) (Top over Timeline, Assignee & definition) Technology over top assignee trends Technology Co-occurrence Matrix provides facility for easily identify the patents / patent filings which are claiming more than one technology categories

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