Biophotonics lecture 9. November 2011. Last time (Monday 7. November). Review of Fourier Transforms (will be repeated in part today) Contrast enhancing techniques in microscopy Brightfield microscopy Darkfield microscopy Phase Constrast Microscopy Polarisation Contrast Microscopy
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Amplitude indicated by brightness
Phase indicated by color
I(k) = A(k) A(-k)
I(x) = |A(x)|2 = A(x) · A(x)*
kyOptical Transfer Function
A microscope is a Fourier-filter!