Analytical Separations. Chromatographic Principle. Chromatographic Separations. Column Chromatography － the stationary phase is held in a narrow tube. － the mobile phase is forced through the tube under pressure or by gravity. Planar Chromatography
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
－the stationary phase is held in a narrow tube.
－the mobile phase is forced through the tube
under pressure or by gravity.
－ the stationary phase is supported on a flat plate
or in the pores of a paper
－ the mobile phase moves through the stationary
phase by capillary action or by gravity.
Solute equilibriates between the mobile phase and the stationary liquid.Partition Chromatography
Solute ions of the opposite charge in the mobile liquid phase are attracted to the resin by electrostatic forces.Ion Exchange Chromatography
－high molecular weight polymers
－large numbers of an ionic functional group
－cation exchange resin
－anion exchange resin
K↑strong tendency for binding Ca+2
takes a very long time.
HETP = A + B / u + C u
High resolution: △ Z↑&W↓