the sensational science behind antioxidants testing facts vs myths of popular antioxidant products n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
The Sensational Science Behind Antioxidants: Testing Facts vs. Myths of popular antioxidant products PowerPoint Presentation
Download Presentation
The Sensational Science Behind Antioxidants: Testing Facts vs. Myths of popular antioxidant products

Loading in 2 Seconds...

play fullscreen
1 / 26

The Sensational Science Behind Antioxidants: Testing Facts vs. Myths of popular antioxidant products - PowerPoint PPT Presentation


  • 144 Views
  • Uploaded on

The Sensational Science Behind Antioxidants: Testing Facts vs. Myths of popular antioxidant products. Yesterday, you broke up into groups and selected a popular “antioxidant-rich” product to endorse. Today you will chemically test the drinks to see if there’s truth to their marketing. .

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'The Sensational Science Behind Antioxidants: Testing Facts vs. Myths of popular antioxidant products' - ilya


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
the sensational science behind antioxidants testing facts vs myths of popular antioxidant products

The Sensational Science Behind Antioxidants: Testing Facts vs. Myths of popular antioxidant products

slide2

Yesterday, you broke up into groups and selected a popular “antioxidant-rich” product to endorse

Today you will chemically test the drinks to see if there’s truth to their marketing.

You are the Research & Development Team and the Marketing Team for your product.

part i the science behind antioxidants
Part I: The science behind antioxidants

Free radicals cause damage by oxidation

Free radical

with unpaired electron

Victims of Oxidation

slide4

O

O

Add 1ste-

Add 2nde-

Peroxide radical

O2-2

Superoxide radical

O2-

Molecular Oxygen

O2

Add two H+

Reactive Oxygen Species

ROS

H

O

O

O

O

O

O

O

H

Water

Hydrogen

peroxide

H2O2

H

H

O

O

H

H

Add 2H+

Add 3rde-

H

H

O

O

H

Hydroxyl

radical

OH

Add 4the-

Hydroxyl ion

OH-

Hydroxyl ion

OH-

free radicals cause damage by oxidation
Free radicals cause damage by oxidation

Oxidation occurs when one substance transfers one or more its electrons to another substance

Electrons lost to

+ H2O

+ ½O2

Catechol

Colorless

Benzoquinone

Yellow-Brown

“Catechol is oxidized by O2to become benzoquinone”

“Catechol loses its electrons to O2to become benzoquinone”

slide6

Phenol Oxidase catalyzes the reaction that changes catechol, in the presence of O2, into benzoquinone

Phenol

Oxidase

+ H2O

+ ½O2

Catechol

Colorless

Benzoquinone

Yellow-Brown

slide7

Phenol Oxidase catalyzes the reaction that changes catechol, in the presence of O2, into benzoquinone

Phenol

Oxidase

X

+ H2O

+ ½O2

+

Antioxidant!

Catechol

Colorless

Benzoquinone

Yellow-Brown

slide9

Phenol Oxidase catalyzes the reaction that changes catechol, in the presence of O2, into benzoquinone

Phenol

Oxidase

+ H2O

+ ½O2

Catechol

Colorless

Benzoquinone

Yellow-Brown

slide10

Can any of these products inhibit the browning reaction in mushrooms?

x 10

Controls:

Buffer

pH 7

100X Daily Dose of Vitamin C

purifying mushroom oxidase

2. Chop mushroom into pieces and put into Ziploc bag

3. Close bag tightly and mash gently with fingers

Purifying mushroom oxidase

1. Weigh mushroom

Multiply mass by 4 and add that amount of buffer to the bag

Perform the tests!

4. Wet the coffee filter with buffer and place over cup. Use pipette to transfer mushroom extract to filter

spot test
Spot test

Time = 0 min

  • Using the pipette, add two drops of mushroom oxidase to every square on your table.
  • Add the appropriate reactants to each square in the order they are listed
  • Once all the drops have been added to the 6 squares, start the timer.
  • Observe the reaction for5 minutes, then answer the following questions.

Hint: Use a steady hand so that each drop is the same amount of liquid. Make sure the paper is level, so the drops do not move.

Time = 5 min

slide13

Double bonds absorb light

http://science-edu.larc.nasa.gov/EDDOCS/Wavelengths_for_Colors.html

slide15

How does a spectrophotometer work?

0.430

Absorbance

Readout

At Abs490 nm:

Wavelength selector

+ H2O

+ ½O2

Prism to disperse light

Light

Catechol

Colorless

Benzoquinone

Yellow-Brown

0.240

0.520

spectrophotometer test
Spectrophotometer Test
  • Label your test tubes #2, #4, #6 and buffer blank
  • Add 4 ml of buffer to “buffer blank”. Place the tube into spectrophotometer holder and use the left knob to set the numbers in the absorbance window to 0.000.
  • Add the reactants for Tube #2—use correct pipettes for each reactant! Mix by tapping the test tube, and place tube into spectrophotometer. Start the timer and record absorbance at t=0 min.
  • Remove tube at t = 4:30 min and mix by tapping test tube. Record absorbance at t= 5 min.
  • Repeat steps 3-4 for each Tubes #4, #6.
there are many different ways to test for antioxidants
There are many different ways to test for antioxidants

Our mushroom oxidase test may not be sensitive enough to pick up on antioxidant levels

slide18

Phenol

Oxidase

X

+ H2O

+ ½O2

+

Antioxidant!

Catechol

Colorless

Benzoquinone

Yellow-Brown

slide19

Phenol

Oxidase

X

+ H2O

+ ½O2

+

Antioxidant!

Catechol

Colorless

Benzoquinone

Yellow-Brown

Your drink has antioxidants, but it may be very low amounts compared to

100X Daily Value of Vitamin C

present a 3 minute marketing campaign that showcases
Present a 3 minute marketing campaign that showcases:
  • Your knowledge on the mechanism and health benefits of antioxidants defense against free radicals.
  • Your product’s antioxidant test results.
  • Your opinion why your classmates should buy your antioxidant drink.
slide22

(Instructor note: show a video clip of antioxidants in the media.

In my presentation, I edited together a video of 9 different commercials, interviews on the topic of antioxidants)

Edited by Ms. Theresa

slide23

Do not make scientific claims that you cannot back with evidence

Find a different approach to market the product

much research has to be performed before claims can be made1
Much research has to be performed before claims can be made

“I recommend pizza since it is a vegetable and therefore has *tons* of antioxidants.”

I’m actually a real doctor! Google me!

slide26

Sample Ballot

Which product is the best antioxidant drink on the market?

The best scientific argument was made by:

The best marketing campaign was for:

☐7-Up Antioxidant

☐ Activate Antioxidants

☐ FUZE Triple Berry

☐ Lipton Green Tea

☐ POM Wonderful

☐ Vitamin Water XXX

☐ Other ___________

☐ 7-Up Antioxidant

☐ Activate Antioxidants

☐ FUZE Triple Berry

☐ Lipton Green Tea

☐ POM Wonderful

☐ Vitamin Water XXX

☐ Other ___________

Write-in: Based on the overall campaign and facts, I would consider buying: