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PROTEIN SYNTHESIS

PROTEIN SYNTHESIS. In this experiment we modeled the structure of DNA and the processes involved in protein synthesis. By: Shelley Quirk. Equipment. 42 toothpicks 18 milk bottles cut in half (36 halves) – sugar 18 raspberry lollies cut in half- phosphate

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PROTEIN SYNTHESIS

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  1. PROTEIN SYNTHESIS In this experiment we modeled the structure of DNA and the processes involved in protein synthesis By: Shelley Quirk

  2. Equipment • 42 toothpicks • 18 milk bottles cut in half (36 halves) – sugar • 18 raspberry lollies cut in half- phosphate • 25 jelly beans cut in half (5 of each 5 colours)- bases: • Adenosine- orange • Thymine- purple • Cytosine- pink • Guanine- green • Uracil- blue • 4 jelly snake, aprox. 6cm long, different colours • A4 white paper representing a cell • Colored paper circle, 6cm diameter- a ribosome • Clean sharp knife • Cutting board • Gloves • Scissors • Marking pen • Heinemann Biology textbook

  3. DNA is a double stranded helix molecule made of subunits called nucleotides. • Each nucleotide contains a sugar (deoxyribose), a phosphate, and a base. • There are four bases (adenosine, thymine, cytosine and guanine). • Alternate sugar and phosphates form the sides with bases connected to the sugars making “rungs” like a ladder.

  4. The information about the number, type and sequence of amino acids, needed to make a protein molecule, is found as a code in DNA. The code- a sequence of bases. One gene sequence codes for one polypeptide (a single chain of many amino acids) A set of 3 bases (a codon) codes for one amino acid of a polypeptide. A protein is one or more polypeptides.

  5. a gene length of DNA unwinds in the nucleus

  6. RNA polymerase enzyme moves along the exposed single DNA strand linking complementary RNA nucleotides together to form a messenger RNA strand. RNA contains the base uracil where thymine is found in DNA.

  7. The mRNA strand is then modified so that it only consists of the base sequence that will code for the protein. It removes the non-coding regions, introns, while still in the nucleus by splicing the coding regions, exons, together. • The modified mRNA then moves from the nucleus into the cytoplasm • The start codon (AUG) and a stop codon control the length of the mRNA strand.

  8. In the cytoplasm, an enzyme attaches amino acids to tRNA molecules. Each type of amino acid is attached to its specific tRNA.

  9. The modified mRNA moves out of the nucleus into the cytoplasm.

  10. TRANSLATION • The start codon (AUG) end of the mRNA strand binds onto a ribosome. A tRNA carrying the amino acid methionine at one end and anticodon (UAC) at the other, binds to the mRNA start codon within the ribosome. DNA strand Amino acid forming polypeptide bond (jelly snakes) Ribosome mRNA strand

  11. A second tRNA binds to the next codon. Its amino acid links to the polypeptide bond of the first amino acid. • The first tRNA is released from the ribosome. The ribosome moves along the mRNA strand one codon at a time. Two tRNAs at a time are temporarily bound within the ribosome and their amino acids linked together DNA strand Amino acid forming polypeptide bond (jelly snakes) Ribosome mRNA strand

  12. When a ‘stop’ codon isreached the polypeptide chain is released into the cytoplasm A polypeptide chain forms (jelly snakes)

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