DNA Science Day 1 Amplifying and Cutting. APh162 Winter 2005 Caltech. So, what’s a plasmid?. What are the tools?. PCR = Xerox Machine Amplify DNA Restriction Enzymes = Scissors Target very specific DNA sequences Ligase = Glue Transformation and DNA extraction. Making a modular plasmid.
Lutz and Bujard (1997)