Chapter 5. Microbial Nutrition. The Common Nutrient Requirements. Macroelements (macronutrients) C, O, H, N, S, P, K, Ca, Mg, and Fe required in relatively large amounts Micronutrients ( trace elements ) Mn, Zn, Co, Mo, Ni, and Cu required in trace amounts
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Study table adapted from Microbiology, Prescott, Chapter Five.
http://biocyc.org/ECOLI/new-image?type=ENZYME&object=GLPF-MONOMER. These proteins exist in plants, animals, fungi, bacteria, and protists.
Microbiol Mol Biol Review. 2000 December; 64 (4): 672–693
José A. Vázquez-Boland,1,2* Michael Kuhn,3 Patrick Berche,4 Trinad Chakraborty,5 Gustavo Domínguez-Bernal,1 Werner Goebel,3 Bruno González-Zorn,1 Jürgen Wehland,6 and Jürgen Kreft3
Selects for Gram –
Differentiates between bacteria based upon fermentation of lactose( color change)
1. S. aureus
Positive - growth
2. S. epidermidis
Negative( color does not change) – no fermentation of mannitol with production of acid
3. M. luteus
http://www.jlindquist.net/generalmicro/102diff.html - General Reference
http://medic.med.uth.tmc.edu/path/macconk.htm - MacConkey Agar
http://www.indstate.edu/thcme/micro/hemolys.html - Blood Agar
1. dispense cells onto
medium in petri dish
4. spread cells
2. - 3. sterilize spreader
Terms1. Colony shape and size: round, irregular, punctiform (tiny)2. Margin (edge): entire (smooth), undulate (wavy), lobate (lobed)3. Elevation: convex, umbonate, flat, raised4. Color: color or pigment, plus opaque, translucent, shiny or dull5. Texture: moist, mucoid, dry (or rough).