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Detection of Psychoactive Compounds in Psilocybe Cyanescens Developmental Stages

Explore the significance of detecting psychoactive drugs in the different growth stages of Psilocybe cyanescens, crucial for clandestine grow operations, law enforcement personnel, and forensic chemists. This study analyzes the presence of Psilocybin and Psilocin in mycelium knots and fruiting bodies through TLC and GC/MS techniques.

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Detection of Psychoactive Compounds in Psilocybe Cyanescens Developmental Stages

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  1. Detecting Psychoactive Drugs in the Developmental Stages of Psilocybe cyanescencs Gross, Susan T. J Forensic Sci 2000;45(3): 527–537.

  2. Why this is important • Clandestine Grow Operations • Law Enforcement Personnel • Forensic Chemists

  3. Introduction • Psilocybe Cyanescens • Psilocybin/Psilocin • Various Stages of Growth

  4. Methods • Mushrooms grown from spore stage in sterile conditions. • Some were grown in dark and some in light • TLC of various extracts • GC/MS of various extracts

  5. Preparation of Samples • Standard Psilocybin and Psilocin obtained • Mycelium Knot Samples soaked in MeOH • All other Extracts cleaned w/ H2SO4, washed with Chloroform. Made Basic, psilocybin/psilocin extracted with Chloroform, and reconstituted with MeOH

  6. Results

  7. Results

  8. Conclusions • Psychoactive Drugs weren’t detected in spores or mycelium • Both Psilocybin and Psilocin were detected in mycelium knots and the fruiting body of the mushroom • Light effects growth of P. cyanescens but not detection or content of psychoactives

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