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Effects of FSP27 Knockdown and Overexpression on FFA Release from Human Adipocytes

This study investigates the impact of FSP27 knockdown and overexpression on the release of free fatty acids (FFA) from human adipocytes. Through a series of experiments, we demonstrate that knocking down FSP27 results in increased FFA release, while overexpressing FSP27 decreases this release. Additionally, we performed co-immunoprecipitation studies to show that FSP27 interacts with both HA- and Myc-tagged Egr1 in HEK293T cells. The results were analyzed using standard statistical methods, highlighting the significance of FSP27 in lipid metabolism regulation.

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Effects of FSP27 Knockdown and Overexpression on FFA Release from Human Adipocytes

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  1. A B Figure S1. Knock down of FSP27 increases while over-expression of FSP27 decreases the release of FFA from human adipocytes. FA were measured after 2.5 h incubation of cells in KRB with 4% BSA. All values are means ± SD; p value was estimated by unpaired t-test. B A Egr1 positive control Egr1 positive control IP: Myc HA Myc IP: HA Myc HA Egr1 Egr1 Myc HA HA-FSP27: + + - Myc-FSP27: + + - Egr1: + + - Egr1: + + - Figure S2. FSP27 co-immunoprecipitates with both HA- and Myc-tagged Egr1. HEK293T cells growing in 65-mm dishes were transfected with 4 µg of HA-tagged FSP27 (panel A) or Myc-tagged FSP27 (panel B) cDNA and 2 µg of Egr1 cDNA. Cell lysates were collected 24 h post-transfection and immunoprecipitated with 2 µg of either HA or Myc antibody and Protein A/G magnetic beads. The eluates were then separated by 12.5% PAGE and immunoblotted using HA, Myc, and Egr1 antibodies as indicated.

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