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Research Techniques Made Simple: Gene Knockout in Mice. Christian Günschmann, Elena Chiticariu, Bhavuk Garg, Merve Meliha Hiz, Yora Mostmans, Maria Wehner, Lukas Scharfenberger. What Is Inducible Mouse Technology?.

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research techniques made simple gene knockout in mice

Research Techniques Made Simple:Gene Knockout in Mice

Christian Günschmann, Elena Chiticariu, Bhavuk Garg, Merve Meliha Hiz, Yora Mostmans, Maria Wehner, Lukas Scharfenberger

what is inducible mouse technology
What Is Inducible Mouse Technology?

Allows the tissue/cell type-specific regulation of gene expression at a time point that is determined by the investigator – spatiotemporal control of gene knockout or gene knockin

confirmation of tamoxifen mediated knockout
Confirmation of Tamoxifen-Mediated Knockout

Concept of fibroblast-specific

inducible TβRII knockout

Loss of TβRII protein confirmed by western blot and IF

wt

Genotyping PCR

Null

Denton et al., J Invest Dermatol 129:194-204, 2009

advantages and limitations of inducible mice

Advantages:

  • Inducible mouse technology enables the investigator to determine where and when to turn on or off genes of interest
  • Allows assessment of the functional importance of overexpression or loss of proteins in normal skin homeostasis and at different stages of disease
  • Limitations:
  • Long-term treatment with the inducer can lead to side effects
  • The system can be “leaky,” resulting in undesired expression of the gene of interest

Advantages and Limitations of Inducible Mice

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