Microbial Diversity in a Dye Treating SBR. by Dr. Naeem ud din Islamia College Peshawar. Biotreatment Different modes. B: isolated organisms. A: using mixed culture. C: isolated enzymes. Dyes are hard to degrade, and often result in harmful intermediates.
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Islamia College Peshawar
A: using mixed culture
C: isolated enzymes
Dyes are hard to degrade, and often result in harmful intermediates
A Specially Designed Airlift BR from a previous Experiment for SND achieving was used
The Nitrogen Removing Process was well established in that Reactor
93 % of Ammonia and COD at an HRT of 12 hrs.
This SBR was subjected to gradually increasing dye concentration
Optimization was achieved at a dye concentration of 25 mg/l and increased HRT of 36 hrs
In this experiment we used the activated sludge as a renewable biological resource to adsorb the usual environmental concentrations of the MG dye.
The Color and COD removal was 80 %
ammonia removal declined to 70 %.
Biomass, 4 + 0.7 to 6 + 0.5 gm/l, SVI was in the range of 30 to 65 ml/gm
UV-Vis spectrophotometric scan of the biodecolorization of malachite green.
Knowledge about the microbial community in a dye treating reactor would be useful in association with operational conditions, to eliminate the pollutants efficiently.
likely to cause the domination of certain groups of bacteria
This aspect inspired our interest to know the microbial community evolved under the selective pressure of the Dye in the SBR.
Bacterial universal primers
27F (3′-AGAGTTTGATCATGGCTCAG-5′) and
1492R (3′-TACGGYTACCTTGTTACGACTT-5′) were used for amplification.
The obtained sequences were edited and aligned using the BioEdit software and CLUSTAL_W program (Thompson, 199724).
The sequences were compared to the known GenBank sequences using Basic Local Alignment Search Tool (BLAST).
Phylogenetic trees were constructed by neighbor-joining method with the MEGA package . Identical sequences were recognized by phylogenetic tree analysis.
If the sequences similarity was more than 97 %, they were considered as identical and used for further phylogenetic analysis as an operational taxonomic unit (OTU).
The isolates Identified with a- & g-Proteobacteria
All these groups well represented
in the polluted environments
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