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Identifying Unknown Carbohydrates: Classification Tests Procedure

This experiment aims to identify unknown carbohydrates (fructose, glucose, maltose, lactose) using sensitive red tetrazolium and Barfoed's tests. Follow lab handout for safety precautions while working with toxic compounds.

herrod-nichols
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Identifying Unknown Carbohydrates: Classification Tests Procedure

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  1. Chapter 63 Carbohydrates

  2. Purpose • In this experiment, unknown carbohydrates (fructose, glucose, maltose, lactose) will be identified using two classification tests. To distinguish between aldehydo- and ketosugars sensitive red tetrazolium (RT) test is employed. If the RT test is positive, Barfoed’s test allows to distinguish between mono- and disaccharides

  3. Comments • Follow pp. 770-771(microscale). • Follow the lab handout for all experimental procedures. Wearing rubber gloves is necessary during this lab. You will work with toxic and corrosive compounds. • Carry out two classification tests to disclose the structure of each unknown carbohydrate.

  4. Safety • Red tetrazolium may be harmful if inhaled, swallowed or absorbed through the skin. Skin, eye and respiratory irritant. Toxicology not fully investigated. Wear protective gloves and goggles. • Copper salts are harmful is swallowed. May cause irritation of respiratory system, do not swallow or inhale dust.

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