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Identifying Unknown Carbohydrates: Experiment with Classification Tests

In this experiment, unknown carbohydrates (fructose, glucose, maltose, lactose) are identified using sensitive red tetrazolium (RT) test to differentiate aldehydo- and ketosugars. Barfoed’s test is used for further classification of mono- and disaccharides. Follow lab guidelines for safety when working with toxic compounds.

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Identifying Unknown Carbohydrates: Experiment with Classification Tests

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  1. Chapter 63 Carbohydrates

  2. Purpose • In this experiment, unknown carbohydrates (fructose, glucose, maltose, lactose) will be identified using two classification tests. To distinguish between aldehydo- and ketosugars sensitive red tetrazolium (RT) test is employed. If the RT test is positive, Barfoed’s test allows to distinguish between mono- and disaccharides

  3. Comments • Follow pp. 770-771(microscale). • Follow the lab handout for all experimental procedures. Wearing rubber gloves is necessary during this lab. You will work with toxic and corrosive compounds. • Carry out two classification tests to disclose the structure of each unknown carbohydrate.

  4. Safety • Red tetrazolium may be harmful if inhaled, swallowed or absorbed through the skin. Skin, eye and respiratory irritant. Toxicology not fully investigated. Wear protective gloves and goggles. • Copper salts are harmful is swallowed. May cause irritation of respiratory system, do not swallow or inhale dust.

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