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Host Defenses. Microbiology 2314. A Healthy Host Has a Variety of Defenses to Prevent Infection. Definitions. Resistance - Ability to ward off disease. Susceptibility - Lack of resistance. Two Types of Resistance. Nonspecific Resistance / Defense Against All Invaders.

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host defenses

Host Defenses

Microbiology 2314

definitions
Definitions
  • Resistance

- Ability to ward off disease.

  • Susceptibility

- Lack of resistance.

nonspecific resistance defense against all invaders
Nonspecific Resistance / Defense Against All Invaders
  • First Line of Defense

1. Skin

2. Mucus Membranes

  • Second Line of Defense

1. Phagocytes

2. Inflammation

3. Fever

specific resistance defense against specific microorganisms
Specific Resistance / Defense Against Specific Microorganisms
  • Antibodies
  • Lymphocytes (B and T Cells)
slide7
Skin

Structure and Composition (Waterproof Keratin) Provide Resistance to Infection.

It is not a great place for bacteria to live…

Dries

Sheds

Secretes

epidermis of the skin
Epidermis of the Skin
  • Epidermis consists of four layers.
  • Top layer is dead
  • 15-40 Rows of Dying Cells
  • Keratin a waterproof protein
  • pH of 3-5

Symbiotic bacteria living on the skin decompose dead skin cells.

The process results in a strong odor as the number of bacteria increase.

slide10
Best Areas for Organisms to Occupy are

Scalp

Ears

Underarms

Genital Regions

Why?

lachrymal apparatus
Lachrymal Apparatus

Protects the eyes from irritating substances and microorganisms.

The conjunctiva has only a small number of bacteria present due to continuous blinking and lachrymal secretions which contain bactericidal substances.

slide12
The lacrimal glands underneath the skin of the upper eyelids make a fluid that is mostly salt and water. This salty water gets to the eye through small openings inside the upper eyelids.
slide13
When the eyelid blinks, the watery liquid is spread across the eye. There are other glands on edges of the eyelids that make oils. The most important of these glands are called the meibomian glands. The oils from these glands actually float on top of the watery fluid in the tears. This keeps the water from evaporating too quickly.
slide14
Some of the oils stay along the edge of the eyelid, and they help keep the tears from "leaking" over the eyelashes. If there is not enough of these oils, tears will keep overflowing from the eyes. Oddly enough, a problem with the meibomian glands can lead to overflowing tears and dry eye syndrome at the same time
slide15
Salivary Glands Produce Saliva

Saliva Washes Microorganisms from Teeth and Gums

slide16
Saliva isn’t always enough.

S. mutans Secretes Sticky Polysaccharide  Plaque

slide18
Tooth decay (dental caries) was not a major problem before the fateful year of 1886. Do you remember why 1886?
european teeth
European Teeth

Teeth in skulls from Europeans prior to the 1500’s showed remarkably well-reserved teeth. Once sugar was introduced into the European diet, teeth deteriorated quickly and tooth decay became a widespread disease.

defensive body secretions
Defensive Body Secretions
  • Tears
  • Saliva
  • Mucus
  • Vaginal Secretions
  • Nasal Secretions
  • Sebum
  • Perspiration
slide22
Mucus traps many microorganisms that enter the respiratory and gastrointestinal tracts.

Snotty noses are a result of the body trying to rid the system of the trapped microorganisms.

slide24
The flow of urine moves microorganisms out of the urinary tract.

The kidney and bladder are usually sterile.

slide25
What is Sebum?

Sebum is an oily/fatty substance secreted from the sebaceous glands (unsaturated fatty acids) that inhibits the growth of pathogenic bacteria.

Constituent % by weight

Glycerides and free fatty acids 57.5

Wax esters 26.0

Squalene 12.0

Cholesteryl esters 3.0

Cholesterol 1.5

perspiration
Perspiration
  • Washes Microorganisms Off Skin
  • Maintains Body Temperature
  • Eliminates Cellular Wastes
  • Contains Lysozyme
  • Lysozyme is Most Effective Against Gram Positive Bacteria
lysozyme
Lysozyme
  • Found In

1. Tears

2. Salava

3. Nasal Secretions

4. Perspiration

the acidity of gastric juice prevents most microbial growth in the stomach
The Acidity of Gastric Juice Prevents Most Microbial Growth in the Stomach
  • Mucus, HCl, Enzymes
  • pH 1.2 - 3.0
  • No effect on Clostridium botulinum
  • No effect on Staphylococcus aureus
ulcers
Ulcers
  • 4 Million Americans Have Ulcers Annually
  • H. pylori is Found in Almost 50% of the Population
  • That 50% Could Either Have Gastritis or Ulcers
why is h pylori able to survive in the acidic environment of the stomach if it isn t an acidophil
Why is H. pylori able to survive in the acidic environment of the stomach if it isn’t an acidophil?
phagocytosis the ingestion of microorganisms or particulate matter by a cell
PhagocytosisThe Ingestion of Microorganisms or Particulate Matter by a Cell

We frequently see this when WBC’s engulf bacteria.

phagocytosis
Phagocytosis
  • Attraction (Chemotaxis)
  • Attachment (Opsonization / Coating with Protein)
  • Ingestion
  • Digestion (Lysosomal Enzymes and Oxidizing Agents)
  • Expulsion
slide38
The Mechanism of Phagocytosis

Chemotaxis is the process by which phagocytes are attracted to microorganisms.

slide41
An accumulation of pus in the front of the eye. In this image, the pus is seen as a pool of whitish fluid between the iris and cornea.
slide44
White Blood Cells (Leukocytes)

Macrophages are Mature Monocytes that are Phagocytic in Nature

Histiocytes are a special type of macrophage fixed in a particular tissue

1. Kupffer Cells / Liver

2. Alveolar macrophages / Lungs

wbc neutrophils
WBC - Neutrophils
  • Increased / Bacterial Infection
  • Normally 50-70
  • Two Types

1. Segmented (Mature)

2. Banded (Less Mature)

wbc basophils
WBC - Basophils
  • Allergic Reactions
  • Leukemias
  • Normally 0-1
wbc eosinophils
WBC - Eosinophils
  • Worm Infections
  • Skin Disorders
  • Scarlet Fever
  • Normally 1-5
wbc lymphocytes
WBC - Lymphocytes
  • Viral Infections
  • German Measles
  • Whooping Cough
  • Syphilis
  • Normally 20-30
wbc monocytes
WBC - Monocytes
  • Recovery from Infections
  • Fungal, Rickettsial, Protozoal, Infections
  • Normally 2-6
differential wbc count 100 cells
Differential WBC Count (100 Cells)
  • Neutrophils 50-70
  • Lymphocytes 20-30
  • Monocytes 2 - 6
  • Eosinophils 1 - 5
  • Basophils 0 - 1

Wright's stain is a technique that is used to make the differences between cells visible under light microscopy.

It is used in the examination of peripheral blood smears and bone marrow aspirates.

slide54
Concentrate your examination in areas where the cells are individually separated from one another and fairly uniformly dispersed
slide56
Neutrophils

Banded Segmented

slide89
Acanthocytes are red blood cells with irregularly spaced projections. These projections vary in width but usually contain a rounded end. They may be found in abetalipoproteinemia and certain liver disorders.
slide90
Auer rods are elongated, bluish-red rods composed of fused lysosomal granules, seen in the cytoplasm of myeloblasts, promyelocytes and monoblasts and in patients with acute myelogenous leukemia.
slide91
Bacteria in the smear. This image was taken from a patient with Streptococcus pnemoniae infection.
slide95
Hairy cells are characterized by their fine, irregular pseudopods and immature nuclear features. They are seen only in hairy cell leukemia.
slide96
Howell-Jolly bodies are spherical blue-black inclusions of red blood cells seen on Wright-stained smears. They are nuclear fragments of condensed DNA, 1 to 2m in diameter, normally removed by the spleen. They are seen in severe hemolytic anemias, in patients with dysfunctional spleens or after splenectomy.
slide97
Nucleated red blood cells, (nrbcs or normoblasts), represent the stages of a red blood cell before it matures. Cells of this stage are usually seen in newborn infants, and in patients with iron deficiency anemia.
case study 1
Case Study 1
  • This 16 year old male came to the emergency room complaining of severe abdominal pain in the right lower quandrant. He had a fever of 101 F. On physical examination, he had a rigid, board-like abdomen and rebound tenderness in the right lower quandrant. There were no other abnormalities.
case study 2
Case Study 2
  • This 15 year old female was sent home from summer camp because of weakness, lassitude, and sore throat. As her family physician, you found that on physical examination she had an inflamed pharynx, enlarged tonsils, several enlarged and slightly tender lymph nodes in the neck, a palpable spleen, and a tender palpable liver edge.
slide101
What is the predominant white blood cell type?

What is your diagnosis in this case?

case study 3
Case Study 3
  • This 65 year old male was in good health except for mild hypertension. At his last check-up, a CBC showed a markedly elevated white blood cell count and physical examination revealed several slightly enlarged lymph nodes in the neck and the axillae, and the spleen was palpable.
case study 4
Case Study 4

This 52 year old male had gradually increasing fatigue together with discomfort in the left upper quandrant. Physical examination revealed an easily palpable spleen and liver edge. A few slightly enlarged lymph nodes were palpable in the neck.

slide106
Inflammation is a bodily response to cell damage.

Inflammation

1. Redness

2. Pain

3. Heat (Local Fever)

4. Swelling

5. Possible Loss of

Function

slide107
Vasodilation

Increase in blood vessel diameter to increase blood flow

1. Histamine / Body Cells

2. Kinins / Blood Plasma

3. Prostaglandin / Damaged Cells

  • Margination

Adherence of phagocytes to vessel walls.

  • Emigration

Phagocytes leaving blood vessels and

entering tissues.

  • Pus

Accumulation of damaged tissue, dead

microbes, and white blood cells (phagocytes).

tissue repair
Tissue Repair
  • Stroma (Supporting Tissue)
  • Parenchyma (Functioning Tissue)
fever
Fever
  • Abnormally High Body Temperature
  • Can Be Induced by Bacterial Endotoxins
  • Chills  Increased Temperature
  • Crisis  Decreasing Temperature
  • Temperature is Controlled by the Hypothalamus
slide114
Do not force food. If diarrhea is present, limit the intake of fruits and juice.
  • Sponging the body with a wet compress, especially keeping it on the forehead, temples and the feet is a very effective method of bringing down the temperature. Use lukewarm water; cool water may cause shivering, which actually raises body temperature. Don't use rubbing alcohol, since it can be toxic. Sponging can be repeated as often as required.
  • Overheating due to overdressing can aggravate the fever so dress lightly, and make sure bedclothes too are light.
  • Temperatures lower than 102 degrees F (38.9 degrees C) usually do not require medication.
  • Give acetaminophen or ibuprofen based on the package recommendations for age or weight. If you don't know the recommended dose call your doctor. Aspirin is not recommended for children under age 12.
slide115
The Complement System

The complement system consists of a group of serum proteins that activate one another to destroy invading microorganisms.

slide116
The complement system helps clear pathogens from an organism. It is derived from many small plasma proteins that form the biochemical cascade of the immune system. Activation of this system leads to cytolysis, chemotaxis, opsonization, immune clearance, and inflammation, as well as the marking of pathogens for phagocytosis. The complement system consists of more than 35 soluble and cell-bound proteins, 12 of which are directly involved in the complement pathways
interferons
Interferons
  • Antiviral Proteins Produced in Response to Viral Infection
  • Alpha
  • Beta
  • Gamma
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