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CsCl centrifugation of DNA over time developed by Meselson and Stahl PowerPoint Presentation
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CsCl centrifugation of DNA over time developed by Meselson and Stahl

CsCl centrifugation of DNA over time developed by Meselson and Stahl

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CsCl centrifugation of DNA over time developed by Meselson and Stahl

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  1. CsCl centrifugation of DNA over time developed by Meselson and Stahl

  2. We will talk about this again in a later lecture: But CsCl gradients are not the same thing as Sucrose Gradients or Agarose Gel Electrophoresis.

  3. CsCl centrifugation of DNA over time

  4. N15is heavier than N14-Can be resolved in CsCl

  5. pulse-chase Experiment: Incubator with N15containing medium for time, then chase with N14medium Expt 1 grows Slowly Expt 2 Bacteria Grow Faster Why?

  6. N14 N15 N14 N15 only only Why would they do 2 different growth rates? Experiment 1 Experiment 2

  7. N14 N15 only Fuse Results from Expt 1 and 2 Cell Divisions

  8. N14 N15 only Experiment 1 observations Watson-Crick Model Does Expt 1 prove hybrid formation?

  9. What about N14/N15hybrid? N15 dsDNA N15 ssDNA Critical Experiment: Hybrid Strand Separation And CsCl centrifugation Looks like control below N15 ssDNA N14 ssDNA