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Detection of Amnionless Protein in Kidney Sections

Detection of Amnionless Protein in Kidney Sections. Authors Natasha Tonge Dr. Roberta Rivi. Acknowledgements. Dr. Roberta Rivi Dr. Sat Bhattacharya Dr. Katia Manova Dr. Betul Altay-Ozer Harlem Children Society Elizabeth Lacy Lab MSKCC. Wax in a Different Context.

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Detection of Amnionless Protein in Kidney Sections

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  1. Detection of Amnionless Protein in Kidney Sections Authors Natasha Tonge Dr. Roberta Rivi

  2. Acknowledgements Dr. Roberta Rivi Dr. Sat Bhattacharya Dr. Katia Manova Dr. Betul Altay-Ozer Harlem Children Society Elizabeth Lacy Lab MSKCC

  3. Wax in a Different Context Tissue sample is embedded in paraffin wax

  4. Wax in a Different Context Tissue sample embedded in paraffin wax Sliced with microtome

  5. Wax in a Different Context Tissue sample embedded in paraffin wax Sliced with microtome Placed in Tissue bath

  6. Wax in a Different Context Tissue sample embedded in paraffin wax Sliced with microtome Placed in Tissue bath Mounted on a slide

  7. Wax in a Different Context Tissue sample embedded in paraffin wax Sliced with microtome http://mskweb3.mskcc.org/MolecularCytology/mccf-final.htm Placed in Tissue bath Mounted on a slide AutomaticallyProcessed

  8. Antibodies in a Different Context Immunohistochemistry - a process used to detect proteins in a tissue section. Antigen - a substance that provokes an immune response Antibody – the protein complex that binds to antigens Enzyme - A protein that speeds up a chemical reaction. Substrate - The chemical that induces the chemical reaction

  9. Antibodies in a Different Context Immunohistochemistry - a process used to detect proteins in a tissue section. Antigen - a substance that provokes an immune response Antibody – the protein complex that binds to antigens Enzyme - A protein that speeds up a chemical reaction. Substrate - The chemical that induces the chemical reaction primary antibody secondary antibody enzyme substrate Antigen

  10. The Anatomy of the Kidney Ammnionless - protein located on the proximal tubule. Proximal Tubule - close to the glomerulus. Cortex - outer region of the kidney. Most likely to find the glomerulus and proximal tubule here. Medula - inner region of the kidney. Lacks ammnionless. http://upload.wikimedia.org/wikipedia/en/thumb/b/b0/Kidney_section.jpg/180px-Kidney_section.jpg altered by Natasha Tonge

  11. The Kidney in Focus (more or less)

  12. The Kidney in Focus (more or less)

  13. The Past and Future of this Experiment To Detect Amnionless in Kidney Sections: • Tissue samples are sectioned, placed on slides and stained • The staining process, immunohistochemistry, makes use of antibodies to induce a color change • That color change enabled us to see the location of the proximal tubules in the kidney sections. In future experiments the same process will be used to pinpoint megalin and cubulin in abnormal mouse tissue. The results will then be accompanied by Western Blotting, Urine analysis and ELISA tests to characterize LRP Y2721-stop mice (mutants with shortened megalin sequences)

  14. Bibliography "Human Hair." Wikipedia. http://en.wikipedia.org/wiki/Human_ hair. 10 July 2006 Barnum, Susan R.. Biotechnology: An Introduction. 2. Belmont, CA: Thomson Brooks/Cole, 2005. Campbell A., Neil, Jane B. Reece, Lawrence G. Mitchell, and Martha R. Taylor.Biology: Concepts & Connections. 4th ed. San Fransisco: Benjamin Cummings, 2003.

  15. owari

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