Update on Biological Small Angle X-ray Scattering (non-crystalline diffraction) program and outcome of the NCRR/BER review. SMB SAC meeting March 28, 2010. SSRL. Hiro T suruta. Hiro Tsuruta (Sr Sci) Thomas Weiss (Staff Sci) Associate staff scientist (vacant since early February, 2010)
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SMB SAC meeting
March 28, 2010
Thomas Weiss (Staff Sci)
Associate staff scientist (vacant since early February, 2010)
Ping Liu (Sci. Prgr)
Lester Carter (PD)
Shared resource for mechanical and electronics developments
Thomas Eriksson (MC) manages the linux PC cluster on BL 4-2.
Support personnel proposed in the BER funding component…Current SMB SAXS/D personnel
Supplements the core NIH NCRR grant
1. Drug target screening for prion deseases (Prusiner, Schoichet, UCSF)
studying promiscuous colloids
high throughput screening for agents modifying PrPC assembly
2. Helps accelerate software developments for HT solution scattering
On-going discussion on collaboration with the Prusiner group
Schoichet very interested
Initiating collaboration with the Mochly-Rosen group (Med School) on PKC
Other targets in discussion (HIV protease, virus capsid assembly)
Dr. Lester Carter joined in January as postdoctoral associate.
Wyatt DLS plate reader delivered in January (characterization experiments)Update: funding opportunities
“Autosampler” version 1.5 in regular use
3 min per a sample/buffer measurement (10 3s exposures + cell cleaning)
<2% failure in sample loading
UV/vis spectrophotomer for in-situ concentration measurements
SolSAXS tab in Blu-Ice
Continuous upgrades of SasTool for real time data processing (WindowsPC + linux)
AbsTool for UV/vis spec data processing
Version 2.0 in assembly
kap95 (NPC), Sali et al.
Recorded with Autosampler
1.7m distanceUpdate: recent achievements in technological developments
Improved beam stability (~10mm level)
LCP sample holder, BL4-2
LCP sandwich plate
LCPRecent achievements (cont.)
High throught lipid phase diagram measurements
User demography FY09 - FY10-1
Average length beam time FY09 & 10-1
1.6 day [can’t do more]
Proposals and users:
50% of all spokespersons (FY09)
Beam time: requested vs scheduled
(FY09 &10#1, excluding Rapid Access)
- Rapid access brings new users.
- Remote access trial successful.
- Cutting down Facility time in FY10-3
- Some of the proposed developments are extension/enhancement or carried-over of the developments in the last grant period.
- Personnel resource limits developments, especially because of strong user program.
SMB SAC discussion needed on these future developments.