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Chromosome 16 Marta Mendes Ignacio Casal Centro de Investigaciones Biológicas August 2012
250 CCD18 150 Proteins to search for 100 75 50 37 25 20 30 mg/lane 15 10 Cell Lines CCD18 MCF7 Ramos 1D SDS PAGE MCF7 RAMOS 250 150 100 75 250 150 50 100 75 37 50 30 mg/lane 30 mg/lane 37 25 20 High throughput analysis 25 Digestion with trypsin 20 15 15 10 10
Equipment : LTQ Orbitrap Velos coupled to a Proxeon nanoLC system LC conditions MS System
High throughput DDA Top15 Proteome discoverer and Sequest with FDR 1% Database: uniprot_sprot_human_HPP KLEEENENLR CCD18 No proteins found MCF7 Q8TBY8-2 RAMOS
Target using global mass list Proteotypic peptides obtained at SRM Atlas LC conditions MS System MAXQUANT Database: uniprot_sprot_human_HPP
Q8TBY8 isoforms alignment Q8TBY8 isoforms 1 and 2 are present in both CCD18 and Ramos cell lines