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Enzymes

Enzymes. What are Enzymes?. Globular proteins Biological catalysts Do not change after reaction They are found in small quantities Specific Reactions are reversible. How Enzymes Work. Enzymes lower the energy required for a chemical reaction to occur (activation energy). How Enzymes Work.

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Enzymes

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  1. Enzymes

  2. What are Enzymes? • Globular proteins • Biological catalysts • Do not change after reaction • They are found in small quantities • Specific • Reactions are reversible

  3. How Enzymes Work • Enzymes lower the energy required for a chemical reaction to occur (activation energy)

  4. How Enzymes Work

  5. Enzyme Structure • Tertiary Structure of enzyme determines its unique shape. • Forms its ACTIVE SITE Active Site

  6. Enzyme Action • Enzymes work on specific substrates • Substrate(s) has to fit in the active site • Lock and key hypothesis Products Substrate Active site Enzyme /Substrate Complex Enzyme unchanged Enzyme

  7. Enzyme Action • Active site is sometimes flexible • When substrate(s) enter active site enzyme changes shape • Induced Fit Theory Product Substrates Active site Enzyme /Substrate Complex Enzyme unchanged Enzyme

  8. Enzyme Action • Rate of an enzyme reaction can be affected by: • Temperature • pH • Enzyme Concentration • Substrate Concentration

  9. Monitoring Enzyme Action • In an experiment enzyme action can be timed by: • Formation of Products e.g. gas • Disappearance of Substrate e.g. starch

  10. Enzyme Activity and Temperature

  11. Enzyme Activity and pH

  12. Enzyme Activity and Substrate Concentration

  13. Enzyme Activity and Enzyme Concentration

  14. What to do now • Read Key Biological Principle box page 77-79 • Answer Checkpoints 2.3 and 2.4

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