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SUPPLEMENTARY DATA Supplementary Figures: 1. Supplementary Figure S1 related to Figure 1

SUPPLEMENTARY DATA Supplementary Figures: 1. Supplementary Figure S1 related to Figure 1 2. Supplementary Figure S2 related to Figure 2 3. Supplementary Figure S3 related to Figure 3 4. Supplementary Figure S4 related to Figure 4 5. Supplementary Figure S5 related to Figure 5

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SUPPLEMENTARY DATA Supplementary Figures: 1. Supplementary Figure S1 related to Figure 1

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  1. SUPPLEMENTARY DATA Supplementary Figures: 1. Supplementary Figure S1 related to Figure 1 2. Supplementary Figure S2 related to Figure 2 3. Supplementary Figure S3 related to Figure 3 4. Supplementary Figure S4 related to Figure 4 5. Supplementary Figure S5 related to Figure 5 Supplementary Tables: 6. Supplementary Table S1 Co-occupied genomic sites of TRIM28 and EZH2 7. Supplementary Table S2 Differentially expressed genes after EZH2 knockdown 8. Supplementary Table S3 Functional annotation of EZH2 Transcriptome 9. Supplementary Table S4 Differentially expressed genes after TRIM28 knockdown 10. Supplementary Table S5 Functional annotation Analysis of TRIM28 Transcriptome 11. Supplementary Table S6 Genes co-regulated by EZH2 and TRIM28 12. Supplementary Table S7 Functional annotation of Co-Activated GeneLists 13. Supplementary Table S8 Peaks of TRIM28 ChIP-seq 14. Supplementary Table S9 TRIM28 ChIP-seq target genes (promoter + genebody) 15. Supplementary Table S10TRIM28 target genes 16. Supplementary Table S11 List of oligonucleotides used for generating mutants. 17. Supplementary Table S12 List of oligonucleotides for RT-qPCR and ChIP analysis 18. Supplementary Figure Legends

  2. Li_FigS1 LC-MS/MS analysis of EZH2-associated proteins in MCF7 cells A B HA Beads Input IgG Mock - - + + - + - - - HA-TRIM28 + + + EZH2 100 130 HA-TRIM28 100 MBP pulldown C GST-TRIM28 GST WT GST-pulldown7 RBCC D MBP-EZH2 + + + + GST-EZH2 (386-746) GST-EZH2 (1-385) WB: GST Input GST GST-TRIM28 (WT) 130 100 130 TRIM28 GST-TRIM28 (RBCC) 75 100 100 GST 75 GST-TRIM28 (WT) 130 50 * * * MBP-EZH2 100 GST-TRIM28 (RBCC) 75 50 37 37 GST (Coomassie Blue Staining) F E

  3. Li_FigS2 A C shEZH2 #1 shEZH2 #2 shEZH2 #3 shEZH2 #4 shEZH2 #5 shEZH2 #6 shCtrl shCtrl EZH2 Actin D B TRIM28 E shCtrl shRNA#1 shRNA#2 TRIM28 EZH2 F TRIM33 Actin G H I

  4. Li_FigS3 A B Screenshot of CXCR4 in EZH2/TRIM28 RNA-Seq/ChIP-seq CXCR4 C Distal primers primers primers primers

  5. Li_FigS4 EZH2 IP IgG Input TRIM28 ARID1A BRM BRG1 SMARCC2 SMARCC1 EZH2

  6. Li_FigS5 A shCtrl shTRIM28 50um 50um B shCtrl shTRIM28 5.3% 1.1% CD44 CD44 CD24 CD24 C

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