Introduction to yeast genetics. Michelle Attner July 24, 2012. What is budding yeast, S. cerevisiae ?. Electron micrograph. DIC (light microscopy). Advantages to budding yeast as a model organism. Simple, eukaryotic cell (~10μm diameter) Compact genome (genome is sequenced)
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July 24, 2012
DIC (light microscopy)
An a and alpha cell can fuse to form an a/alpha diploid.
Yeast have 2 matings types: a and alpha
a haploids and alpha haploids divide
a/alpha diploids can divide asexually or they can undergo meiosis to form four haploid gametes called spores
The goal of mitosis is to produce two daughter cells genetically identical to the mother
Phases of the cell cycle
G1: Gap 1
S: DNA replication
G2: Gap 2
What happens during G1 and G2?
Note that bud size gives you an indication of where the cell is in the cell cycle
Tubulin immunofluorence of an anaphase cell
Actin stained with phalloidin
Journal of Cell Biology
Starvation induces sporulation in yeast
In yeast, scientists can manipulate all four spores that are the products of one diploid cell undergoing meiosis
Marston et al., 2004
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