1 / 4

Neurogenesis

Neurogenesis. Neural stem cells (NSC) were extracted from the subgranular zone of the dentate gyrus of the hippocampus of adult mice. NSC were induced to proliferate → “ Neurosphere ” formed Addition of Test compounds: Saline / Harmine / Tetrahydroharmine (0.5µM).

georgiac
Download Presentation

Neurogenesis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Neurogenesis • Neural stem cells (NSC) were extracted from the subgranular zone of the dentate gyrus of the hippocampus of adult mice. • NSC were induced to proliferate → “Neurosphere” formed • Addition of Test compounds: Saline / Harmine / Tetrahydroharmine (0.5µM). • Assessment of capacity of compounds to promote: • Early stages of Neurogenesis (young neurons): TuJ1 staining (green) • Neuronal Maturation (mature neurons): MAP-2 staining (red)

  2. Distal region of Neurosphere No effects after saline • Color code • Blue staining: cell nuclei (marks all cells) • Green staining: young neurons • Red staining: mature neurons

  3. Harmine Stimulates Neurogenesis in vitro… • Color code • Blue staining: cell nuclei (marks all cells) • Green staining: young neurons • Red staining: mature neurons

  4. …and so does THH • Color code • Blue staining: cell nuclei (marks all cells) • Green staining: young neurons • Red staining: mature neurons

More Related