Translation. mRNA exits the nucleus through the nuclear pores In the cytoplasm, it joins with the other key players to assemble a polypeptide. The other parts of the machinery are: t-RNAs and ribosomes.
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t-RNAs and ribosomes
T-RNA: small, 80n, single strand with secondary structure (folding). Anticodon at end of loop.
function = picks up aa & transports it to ribosome
-Small ribosomal sub units binds to mRNA.
-Initiator tRNA binds to P site
-Large ribosomal unit binds to complex
1. LacZ: codes for B-galactosidase: degrades lactose
2. LacY: codes for B-galactosidase permease: causes lactose to enter the cell
3. LacA: codes for a transacetylase: function unknown!