Translation. Function of 5 ′ CAP. Protect mRNA from degradation-RNAses cannot cleave triphosphate linkages Enhance the translatability of mRNAs-cap needed for binding of cap binding protein which is needed for attachment to ribosome Enhance the transport of the mRNA from nucleus to cytoplasm
between the anticodon and the aa, allowing for
precise positioning of the aa during translation.
1. EF-Tu with the help of GTP, binds an aminoacyl-tRNA to the A site by specific base-pairing of its anticodon and the complementary mRNA codon.
2. Peptidyl transferase forms a peptide bond between the peptide in the P site and the newly arrived aminoacyl- tRNA in the A site.
3. EF-G, with GTP translocates the growing peptidyl-tRNA, with its mRNA codon to the P site. leaving the A site free for the next aminoacyl-tRNA.
Elongation in eukaryotes is carried out with two
elongation factors: eEF-1 and eEF-2:
Mediated in two ways: