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Genetic Recombination at MYH9 Locus: In Vivo and In Vitro Analysis

This study examines recombination events at the MYH9 locus using techniques such as Southern blotting and PCR in both in vivo and in vitro settings. The targeted alleles, MYH9Flx and MYH9Δ, are analyzed for cre recombination. Results show 6.6 kb and 8.3 kb products with specific BamHI, EcoR1, and XbaI sites identified. Understanding these events sheds light on genetic modifications at the MYH9 locus. 8 Relevant

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Genetic Recombination at MYH9 Locus: In Vivo and In Vitro Analysis

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  1. Exon 1 a BamHI EcoR1 XbaI BamHI i MYH9 locus probe 6.6 kb in vivo recombination BamHI BamHI EcoR1 BamHI NEO ii Targeted allele 8.3 kb in vitro cre recombination MYH9Flx iii in vivo cre recombination MYH9Δ iv b. Southern Blot c. PCR +/+ +/- -/- ES +/+ ES +/- 8.3 kb 6.6 kb MYH9Flx wt Supplemental figure S1

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