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This study explores the efficacy of protein capture in Arabidopsis leaf extracts using CPLL eluate at varying pH levels; pH 7 and 9 show higher efficiency when compared to pH 4. Findings are detailed in Supplemental Figure 1.
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Arabidopsis Crude extract pH 9 Arabidopsis Crude extract pH 7 Arabidopsis Crude extract pH 4 Arabidopsis CPLL eluate pH 9 Arabidopsis CPLL eluate pH 7 Arabidopsis CPLL eluate pH 4 226 490 257 449 442 463 567 346 85 Supplemental Figure 1. Application of CPLL to Arabidopsis leaf extracts at pH 4, 7 and 9. Protein capture is more efficient at pH 7 and 9 than at pH 4.