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Factors To Consider When Purchasing A Thermal Cycler Machine
A laboratory that specializes in DNA amplification and polymerase chain reactions is incomplete without a thermal cycler. Also known as thermo cyclers or PCR machines, thermal cyclers are an indispensable tool in the field of molecular biology.
The machine consists of a heating block that contains depressions in which the test tubes are placed. The samples undergo a step by step process of initial denaturation, annealing, extension, final extension, and holding.
The important function of the cycler is to change the temperature of its interior container at specific times for particular durations. These temperature changes allow for the creation of multiple copies of the DNA fragment.
These are available in various models and sizes, ranging from mini, personal models to compact and even large sized machines. For extremely large systems, thermal cycler manufacturers link many PCR machines together with a centralized computer.
As one can imagine, the applications for thermal cyclers machines are numerous. They can be used in life science research, pharmaceutical engineering, animal diagnostics, medicine, and food testing, amongst others.
Type of Vessel and Capacity: The heating blocks in thermal cyclers are designed to store a number of DNA samples. The block can be designed to house DNA samples in small and large capacities up to 300+ samples. The most common container type is for the 0.2 ml PCR tubes.
However, there are other containers designed for 0.5ml or even large conical shaped tubes. The metal used to fabricate the heating block also makes a difference to the operation. Silver is the most preferred metal as it conducts heat and cold better than most metals.
Block Temperature: The precision with which the thermal cycler changes temperature in the heating block is paramount to its successful use. The temperature needs to change uniformly and quickly to provide accurate results.
Check whether the PCR machine has a Peltier element for temperature control. This will confirm that machine will be able to instantaneously change temperatures during the DNA amplification process.
Heated Top Cover: Many a time, the PCR Thermal Cycler process results in the reaction fluids evaporating and creating condensation on the top of the sample lids. This can lead to insufficient data and results, causing a great delay in the work.
Versatility: Research and testing has helped manufacturers to provide a number of flexible solutions, which has made the thermo cycler more versatile in its use. Early versions of it had one solid heating block. Today, the blocks are designed to be interchangeable, enabling for faster DNA amplification of vials of various sizes.
Another option offered to customers is modular heating blocks. This option allows for the interchanging of a single, large heating block with two smaller blocks. Some thermal cyclers also allow the technician to control the temperature of various areas of the block separately.
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